{"id":"W4366998803","doi":"10.1038/s41551-023-01032-2","title":"Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes","year":2023,"lang":"en","type":"article","venue":"Nature Biomedical Engineering","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":128,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Allergy and Infectious Diseases; University of California, San Francisco; National Center for Advancing Translational Sciences; Fondation ARC pour la Recherche sur le Cancer; Canadian Institutes of Health Research; Innovative Genomics Institute; Simons Foundation; CIHR Skin Research Training Centre; Cancer Research Institute; Parker Institute for Cancer Immunotherapy; National Cancer Institute; U.S. Department of Health and Human Services; National Institutes of Health; Government of Canada","keywords":"CRISPR; Ribonucleoprotein; Genome editing; Electroporation; Trans-activating crRNA; Biology; Genome engineering; Molecular biology; Gene delivery; Cas9; Homology directed repair; Transfection; Peptide; Guide RNA; Cell biology; Computational biology; Gene; RNA; Genetics; Biochemistry; DNA repair","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003081801,0.0001403875,0.000191082,0.0001153297,0.00004358233,0.000005226884,0.000230656,0.0002839281,0.00000449897],"category_scores_gemma":[0.0004109546,0.000109606,0.0001328797,0.0002919003,0.0000738035,0.000001564168,0.000112868,0.0001546859,8.48969e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001738397,"about_ca_system_score_gemma":0.00004747191,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005822265,"about_ca_topic_score_gemma":7.645403e-7,"domain_scores_codex":[0.9990185,0.000007003674,0.0002819327,0.0001960748,0.0002407462,0.0002557645],"domain_scores_gemma":[0.999384,0.0001816397,0.00006228465,0.0002156263,0.0000880636,0.00006835895],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001501297,0.00002338474,0.00005825988,0.0002862491,0.00007998241,0.000001383534,0.00004620038,0.01647163,0.978931,0.00008857989,0.002992133,0.001006164],"study_design_scores_gemma":[0.0009230655,0.0001897806,0.005085849,0.0001266415,0.00005192313,0.000006234937,0.0001176291,0.05520414,0.9003864,0.000004023996,0.03768549,0.0002188358],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.91238,0.01296032,0.0722586,0.0002066199,0.001360228,0.0004610835,0.0001754445,0.0001125866,0.00008512836],"genre_scores_gemma":[0.9979428,0.0001029615,0.001116121,0.00003435364,0.0004569832,0.00002692328,0.000252593,0.00002888325,0.00003838186],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08556281,"threshold_uncertainty_score":0.4469602,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005084441370596367,"score_gpt":0.2687178265762187,"score_spread":0.2636333852056224,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}