{"id":"W4366998803","doi":"10.1038/s41551-023-01032-2","title":"Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes","year":2023,"lang":"en","type":"article","venue":"Nature Biomedical Engineering","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":128,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Allergy and Infectious Diseases; University of California, San Francisco; National Center for Advancing Translational Sciences; Fondation ARC pour la Recherche sur le Cancer; Canadian Institutes of Health Research; Innovative Genomics Institute; Simons Foundation; CIHR Skin Research Training Centre; Cancer Research Institute; Parker Institute for Cancer Immunotherapy; National Cancer Institute; U.S. Department of Health and Human Services; National Institutes of Health; Government of Canada","keywords":"CRISPR; Ribonucleoprotein; Genome editing; Electroporation; Trans-activating crRNA; Biology; Genome engineering; Molecular biology; Gene delivery; Cas9; Homology directed repair; Transfection; Peptide; Guide RNA; Cell biology; Computational biology; Gene; RNA; Genetics; Biochemistry; DNA repair","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003288256,0.0002082386,0.000277617,0.000159184,0.000195445,0.0005651272,0.0002575832,0.0004065378,0.002511584],"category_scores_gemma":[0.0001994654,0.0001860948,0.0001943756,0.0001482724,0.0001989996,0.0001300368,0.0002949782,0.000890194,0.001002172],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002461155,"about_ca_system_score_gemma":0.000293221,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003349555,"about_ca_topic_score_gemma":0.0007402199,"domain_scores_codex":[0.9997076,0.00005692273,0.00002118849,0.00006847105,0.00008007428,0.00006564788],"domain_scores_gemma":[0.9998914,0.00003455782,0.00001786286,0.00001839196,0.00001060173,0.00002715933],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006051431,0.00001263617,0.00004113979,0.00002360923,0.000003382755,0.00005013532,0.00001361172,0.0001174658,0.996389,0.0001686487,0.0001373152,0.002982695],"study_design_scores_gemma":[0.00002002304,0.0001173867,0.0004289235,0.000003133532,0.000008707377,0.0002880604,0.000008713781,0.000812464,0.9932458,0.00006378548,0.004999457,0.000003505746],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8595994,0.003831179,0.1214935,0.0004961033,0.0002387794,0.0004243552,0.001486712,0.001005143,0.01142484],"genre_scores_gemma":[0.9677288,0.0008758852,0.02240827,0.0001074687,0.00002370675,0.0000913951,0.0007790463,0.0000902129,0.007895245],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002511584,"threshold_uncertainty_score":0.008402109,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005084441370596367,"score_gpt":0.2687178265762187,"score_spread":0.2636333852056224,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}