{"id":"W4367368962","doi":"10.1186/s13007-023-01009-x","title":"A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants","year":2023,"lang":"en","type":"article","venue":"Plant Methods","topic":"Genomics and Phylogenetic Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Biotechnology and Biological Sciences Research Council; Kanagawa University; James Hutton Institute; Sibbald Trust; Japan Society for the Promotion of Science; Scottish Government; Institute of Genetics; Rural and Environment Science and Analytical Services Division; Sumitomo Foundation","keywords":"Biology; Genome; DNA sequencing; DNA; DNA extraction; Extraction (chemistry); Computational biology; Gene; Genetics; Chromatography; Polymerase chain reaction; Chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009843268,0.001041881,0.0008084868,0.001084181,0.0007307905,0.0006769268,0.0009073297,0.0007326835,0.003381414],"category_scores_gemma":[0.001969139,0.0006232475,0.0007280459,0.001168053,0.0004239167,0.000475451,0.0005554344,0.001603636,0.003491043],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005278239,"about_ca_system_score_gemma":0.001034882,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001882161,"about_ca_topic_score_gemma":0.003858735,"domain_scores_codex":[0.9988575,0.000140848,0.0001124829,0.0003385829,0.0004484708,0.0001021791],"domain_scores_gemma":[0.9992957,0.0001379971,0.000146278,0.00009139843,0.0002654234,0.00006318402],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007313601,0.00004321086,0.0004405557,0.0002851248,0.00001528716,0.000141101,0.0001170635,0.0003680121,0.9783927,0.0002096332,0.001127179,0.01878708],"study_design_scores_gemma":[0.0000360109,0.000313107,0.00663359,0.00008395236,0.00009197196,0.0006539168,0.00008212744,0.005928138,0.9453849,0.0002834368,0.04044154,0.00006722495],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2063538,0.002541783,0.7588225,0.000801754,0.0003182053,0.002889781,0.01450686,0.006262755,0.007502648],"genre_scores_gemma":[0.1205574,0.001760984,0.8182698,0.0004608524,0.0001121612,0.002512769,0.04633337,0.001241339,0.008751285],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003381414,"threshold_uncertainty_score":0.01131189,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06074387987280093,"score_gpt":0.389209472189038,"score_spread":0.328465592316237,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}