{"id":"W4367624317","doi":"10.1021/acsomega.2c08104","title":"Identification of the Neoaspergillic Acid Biosynthesis Gene Cluster by Establishing an <i>In Vitro</i> CRISPR-Ribonucleoprotein Genetic System in <i>Aspergillus melleus</i>","year":2023,"lang":"en","type":"article","venue":"ACS Omega","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Food and Agriculture; University of Kansas; Canadian Institute for Advanced Research; National Institute of Allergy and Infectious Diseases; Division of Intramural Research, National Institute of Allergy and Infectious Diseases; H. L. Snyder Medical Foundation; U.S. Department of Agriculture; National Aeronautics and Space Administration","keywords":"Gene cluster; Biology; Biochemistry; Gene; Yeast; Aspergillus; Biosynthesis; Microbiology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009974496,0.0002684732,0.0005099986,0.0003645489,0.0001122583,0.00007434623,0.0004807492,0.0002302028,0.000006655587],"category_scores_gemma":[0.00028259,0.0001904495,0.0001244441,0.001886373,0.0001063025,0.0002650905,0.0001713513,0.0003052818,0.00004457744],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001933992,"about_ca_system_score_gemma":0.00006285541,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007567143,"about_ca_topic_score_gemma":0.0001332114,"domain_scores_codex":[0.9973436,0.0002451319,0.0008589848,0.0006334653,0.0004378495,0.0004809728],"domain_scores_gemma":[0.9984797,0.00009743116,0.0002622247,0.000945672,0.0001126378,0.0001023085],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001635452,0.0001102629,0.0005217791,0.0002848232,0.00002272943,0.00002121117,0.0002574455,0.00003532377,0.9898698,0.00001194756,0.001312799,0.007388388],"study_design_scores_gemma":[0.0006879785,0.0000423661,0.003975498,0.0002597809,0.00006202775,0.00003282614,0.0003813843,0.0007726164,0.9917025,0.00001542237,0.001855046,0.0002125691],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935817,0.001106808,0.000009022482,0.003679711,0.0003327003,0.0009796455,0.0001178753,0.00009816734,0.00009441462],"genre_scores_gemma":[0.9985418,0.0002138747,0.0001779669,0.0004087087,0.0001518017,0.0000153585,0.00005097494,0.00005711881,0.0003824342],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007175819,"threshold_uncertainty_score":0.7766305,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008651325144815645,"score_gpt":0.2217841527090378,"score_spread":0.2131328275642222,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}