{"id":"W4367624317","doi":"10.1021/acsomega.2c08104","title":"Identification of the Neoaspergillic Acid Biosynthesis Gene Cluster by Establishing an <i>In Vitro</i> CRISPR-Ribonucleoprotein Genetic System in <i>Aspergillus melleus</i>","year":2023,"lang":"en","type":"article","venue":"ACS Omega","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Food and Agriculture; University of Kansas; Canadian Institute for Advanced Research; National Institute of Allergy and Infectious Diseases; Division of Intramural Research, National Institute of Allergy and Infectious Diseases; H. L. Snyder Medical Foundation; U.S. Department of Agriculture; National Aeronautics and Space Administration","keywords":"Gene cluster; Biology; Biochemistry; Gene; Yeast; Aspergillus; Biosynthesis; Microbiology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001811267,0.0004688161,0.0003235043,0.000393546,0.000185395,0.0004297102,0.000422125,0.0003733319,0.0008744636],"category_scores_gemma":[0.0001715833,0.0002325393,0.0005398458,0.0003080895,0.0002474423,0.0001448622,0.000378582,0.0006660821,0.0009189896],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004701685,"about_ca_system_score_gemma":0.0004485481,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002089173,"about_ca_topic_score_gemma":0.002983026,"domain_scores_codex":[0.9998152,0.00002092168,0.00003115287,0.00005243741,0.00005218653,0.00002814275],"domain_scores_gemma":[0.9998376,0.00002539525,0.00006938801,0.00002144008,0.00001716652,0.00002891943],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004783498,0.00001491529,0.000191907,0.00003267271,0.000003967866,0.00007912004,0.00002337181,0.0001876156,0.9979941,0.0001367008,0.00004983095,0.00123817],"study_design_scores_gemma":[0.00001993121,0.0001151551,0.004396519,0.00002195949,0.00003327082,0.0003096838,0.00006521737,0.003259995,0.984263,0.0001055014,0.007392871,0.00001671734],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9296401,0.0008367501,0.05516031,0.000426037,0.0001087649,0.0003599926,0.008083386,0.0009634931,0.00442124],"genre_scores_gemma":[0.9192638,0.0008655401,0.06317759,0.0002006583,0.00001648778,0.0001974551,0.00943255,0.000335246,0.006510647],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002089173,"threshold_uncertainty_score":0.004154086,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008651325144815645,"score_gpt":0.2217841527090378,"score_spread":0.2131328275642222,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}