{"id":"W4376129886","doi":"10.1101/2023.05.10.540076","title":"Universal method for the gentle isolation of intact microvessels from frozen tissue: a multiomic investigation into the neurovasculature","year":2023,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Barrier Structure and Function Studies","field":"Neuroscience","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Jewish General Hospital; McGill University; Douglas Mental Health University Institute","funders":"Canadian Institutes of Health Research; Canada First Research Excellence Fund; Fondation Brain Canada","keywords":"Transcriptome; Pathology; Pericyte; Homogenization (climate); Cell biology; Centrifugation; Biology; Isolation (microbiology); Neuroscience; Medicine; In vitro; Endothelial stem cell; Gene; Bioinformatics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001219708,0.0009203767,0.0005898246,0.001138347,0.0007764224,0.0007238163,0.0006607581,0.0005379008,0.001779086],"category_scores_gemma":[0.000737424,0.0003721235,0.0004982679,0.0007757166,0.0007425967,0.000454736,0.001106484,0.001289034,0.001750886],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002764329,"about_ca_system_score_gemma":0.0006363225,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004392576,"about_ca_topic_score_gemma":0.001259112,"domain_scores_codex":[0.9991861,0.0001241677,0.00009016989,0.0002983519,0.000186499,0.0001146624],"domain_scores_gemma":[0.9995542,0.00007481974,0.00007928311,0.0001356318,0.0001069052,0.00004915332],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003883542,0.00002548833,0.000274697,0.00008611634,0.00001656385,0.00007421477,0.0000603413,0.00005403102,0.9950736,0.0003553797,0.0003415969,0.003599148],"study_design_scores_gemma":[0.00002515391,0.0001894934,0.008696754,0.00008209058,0.00008511813,0.0007865259,0.00007661513,0.001939396,0.9620108,0.0007224678,0.02534797,0.00003761658],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2305652,0.004885845,0.7479013,0.0005461231,0.0005172925,0.001878857,0.006265576,0.002515176,0.004924581],"genre_scores_gemma":[0.2065112,0.004295009,0.7580673,0.0005960095,0.0001725747,0.00496862,0.01490907,0.001375792,0.00910446],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001779086,"threshold_uncertainty_score":0.006450474,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03060632104461208,"score_gpt":0.2606108077356988,"score_spread":0.2300044866910868,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}