{"id":"W4376272836","doi":"10.1002/adma.202303370","title":"Induction of Bleb Structures in Lipid Nanoparticle Formulations of mRNA Leads to Improved Transfection Potency","year":2023,"lang":"en","type":"article","venue":"Advanced Materials","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":210,"is_retracted":false,"has_abstract":true,"ca_institutions":"FPInnovations; Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; National Science Foundation","keywords":"Transfection; Messenger RNA; Potency; In vitro; In vivo; Bleb (medicine); Intracellular; Biophysics; Chemistry; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003889648,0.000373217,0.000309737,0.0002061135,0.0001583406,0.0004183225,0.0001860831,0.000468638,0.0008344316],"category_scores_gemma":[0.0006189006,0.0002096565,0.0002297701,0.0001314666,0.0004613173,0.0003254148,0.0002524694,0.0006982507,0.0004608439],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004894441,"about_ca_system_score_gemma":0.0002463475,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000334349,"about_ca_topic_score_gemma":0.0006306099,"domain_scores_codex":[0.9997267,0.000073316,0.00003778017,0.0000553866,0.00006363157,0.00004318452],"domain_scores_gemma":[0.9996164,0.0001671845,0.0001026096,0.00002821618,0.00005266582,0.00003292969],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001812883,0.00000416924,0.00001634726,0.00001649221,9.205866e-7,0.00001050561,0.000007907625,0.00005455866,0.9995363,0.00003648191,0.000007293862,0.0002908873],"study_design_scores_gemma":[0.000002247403,0.00004933271,0.00008812406,0.000001694686,0.000001841456,0.00001439006,0.000002358302,0.0001879998,0.9993331,0.00001019396,0.0003072475,0.000001492374],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9697176,0.001259525,0.02547173,0.0003179517,0.00005139341,0.0001219413,0.0001388884,0.0002806101,0.002640372],"genre_scores_gemma":[0.9794112,0.0008605827,0.01543363,0.0001339759,0.00001261321,0.00009638956,0.0002276951,0.00009166269,0.003732288],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008344316,"threshold_uncertainty_score":0.003551185,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01132788403523171,"score_gpt":0.2722488461444323,"score_spread":0.2609209621092006,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}