{"id":"W4376272836","doi":"10.1002/adma.202303370","title":"Induction of Bleb Structures in Lipid Nanoparticle Formulations of mRNA Leads to Improved Transfection Potency","year":2023,"lang":"en","type":"article","venue":"Advanced Materials","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":210,"is_retracted":false,"has_abstract":true,"ca_institutions":"FPInnovations; Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; National Science Foundation","keywords":"Transfection; Messenger RNA; Potency; In vitro; In vivo; Bleb (medicine); Intracellular; Biophysics; Chemistry; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001091014,0.00006736661,0.0001266202,0.00007673249,0.00001674379,0.000004163801,0.00005999998,0.00006263425,0.00002034181],"category_scores_gemma":[0.00005448286,0.0000648347,0.00002846155,0.000157328,0.00001645061,0.0000107574,0.00002196296,0.00001664668,0.000003587841],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007787598,"about_ca_system_score_gemma":0.00001984478,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007368669,"about_ca_topic_score_gemma":0.00008788978,"domain_scores_codex":[0.9994059,0.00002313743,0.0002532212,0.0001423899,0.00005792969,0.0001174553],"domain_scores_gemma":[0.9996967,0.00000439618,0.00005917218,0.0001387676,0.00008090196,0.00002007774],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001756753,0.0000157422,0.0001721616,0.00002606884,0.000006971321,1.265634e-7,0.00008728312,0.001143039,0.995127,0.00005604312,0.00002611316,0.003163822],"study_design_scores_gemma":[0.0003002379,0.0003552351,0.01040171,0.00001602487,0.000005360534,9.719378e-7,0.00004606484,0.000007693582,0.9882011,0.00048149,0.0001189852,0.00006506177],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9991279,0.00001733115,0.0002715586,0.00004636116,0.0002757334,0.0001802876,0.00003617744,0.000009190165,0.00003542273],"genre_scores_gemma":[0.9993021,0.00006510822,0.0004138953,0.00002222852,0.00005122101,0.00002434005,0.00004526984,0.000008439661,0.00006732491],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01022955,"threshold_uncertainty_score":0.2643883,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01132788403523171,"score_gpt":0.2722488461444323,"score_spread":0.2609209621092006,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}