{"id":"W4376618108","doi":"10.1101/2023.05.13.540667","title":"Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of <i>Treponema pallidum</i> BamA (TP0326)","year":2023,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Syphilis Diagnosis and Treatment","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of Toronto","funders":"National Institute of Allergy and Infectious Diseases; Connecticut Children's Medical Center; University of Connecticut Health Center","keywords":"Bama; Biology; Epitope; Monoclonal antibody; Bacterial outer membrane; Pilin; Treponema; Virology; Molecular biology; Phage display; Ectodomain; Epitope mapping; Idiotype; Antigen; Microbiology; Antibody; Virulence; Genetics; Receptor; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002142253,0.0004749136,0.0001740433,0.0001906897,0.0001020873,0.0002779798,0.0003384211,0.0003371361,0.000572671],"category_scores_gemma":[0.0001614606,0.0001613214,0.0002451623,0.0001089385,0.0001633126,0.000159591,0.0003459479,0.0006942744,0.0002513924],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002017153,"about_ca_system_score_gemma":0.000116998,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004129077,"about_ca_topic_score_gemma":0.0005192338,"domain_scores_codex":[0.9998092,0.0000314819,0.00001298016,0.00004075402,0.00005358936,0.00005191415],"domain_scores_gemma":[0.9999045,0.00002081396,0.00002145179,0.00001449367,0.00001735059,0.00002127458],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002517835,0.00003992974,0.00007711809,0.000006747616,0.00000204377,0.00002002813,0.000007348263,0.00005007843,0.998968,0.00005232413,0.00001252027,0.0007388472],"study_design_scores_gemma":[0.00001058718,0.0003420434,0.001086134,0.00000162628,0.000006858862,0.000120545,0.000007199489,0.001503415,0.9961364,0.00001413648,0.0007678687,0.000003218432],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9850224,0.0001078349,0.01325554,0.00003951537,0.00001748091,0.00006791823,0.00008746559,0.00007566817,0.001326208],"genre_scores_gemma":[0.9851009,0.0001272106,0.01165233,0.00007210657,0.00000537789,0.00005171035,0.0004045347,0.00002089626,0.002564841],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000572671,"threshold_uncertainty_score":0.001915812,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03915160488683871,"score_gpt":0.2676137138311742,"score_spread":0.2284621089443355,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}