{"id":"W4376649147","doi":"10.1016/j.pep.2023.106295","title":"A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing","year":2023,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Wilfrid Laurier University; McGill University","funders":"Canadian Institutes of Health Research; Canada Research Chairs","keywords":"HEK 293 cells; Transfection; Bioreactor; Cell culture; Recombinant DNA; Laboratory flask; Suspension (topology); Yield (engineering); Chemistry; Cell biology; Chromatography; Biology; Molecular biology; Biochemistry; Materials science; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001842256,0.001510745,0.002047837,0.001363886,0.0008066515,0.001203569,0.001399574,0.00121754,0.005971028],"category_scores_gemma":[0.001066349,0.0008779446,0.001475746,0.001110923,0.0005373494,0.0006589549,0.001210629,0.002890541,0.01295336],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004891686,"about_ca_system_score_gemma":0.0007197552,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005340994,"about_ca_topic_score_gemma":0.0008342444,"domain_scores_codex":[0.9981018,0.0002593435,0.0002760073,0.0004337044,0.0007635254,0.0001656015],"domain_scores_gemma":[0.9994445,0.0001111944,0.00006363757,0.0001545739,0.0001807773,0.00004535377],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007481124,0.0001334346,0.0001198312,0.0003178833,0.00002115501,0.00020356,0.00008612667,0.0001252294,0.9853157,0.0004335996,0.002279481,0.01088928],"study_design_scores_gemma":[0.0000586298,0.0003494336,0.00130372,0.00006126364,0.0000815091,0.001076589,0.00004183055,0.001698565,0.9169351,0.000395062,0.07792211,0.00007621889],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.05616787,0.00494378,0.9012818,0.0007408086,0.0008564997,0.004656013,0.0122333,0.0103493,0.00877064],"genre_scores_gemma":[0.09334338,0.006102381,0.8279267,0.0006023613,0.000215063,0.01099775,0.03999717,0.002190453,0.01862474],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.005971028,"threshold_uncertainty_score":0.01997501,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01776973592706278,"score_gpt":0.2469747422575829,"score_spread":0.2292050063305201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}