{"id":"W4376649147","doi":"10.1016/j.pep.2023.106295","title":"A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing","year":2023,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Wilfrid Laurier University; McGill University","funders":"Canadian Institutes of Health Research; Canada Research Chairs","keywords":"HEK 293 cells; Transfection; Bioreactor; Cell culture; Recombinant DNA; Laboratory flask; Suspension (topology); Yield (engineering); Chemistry; Cell biology; Chromatography; Biology; Molecular biology; Biochemistry; Materials science; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002658912,0.0002614809,0.0001789168,0.0001347579,0.0004077003,0.0001186788,0.0000965293,0.0001967255,0.0000118802],"category_scores_gemma":[0.0001488611,0.0002167905,0.00005457652,0.0001647661,0.00004188895,0.00003365034,0.00007067114,0.00009793717,0.000004500969],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001548314,"about_ca_system_score_gemma":0.00006339783,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001749324,"about_ca_topic_score_gemma":0.000009010307,"domain_scores_codex":[0.9983239,0.00006620117,0.0003050181,0.0008147464,0.0001656615,0.0003244272],"domain_scores_gemma":[0.9992114,0.000007418305,0.0001494899,0.0002924999,0.0001433372,0.0001958412],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004658051,0.0001134624,0.0000368205,0.0003967669,0.0000107316,5.166766e-7,0.0001403756,0.00001515975,0.9609185,0.000007031289,0.002892105,0.03500267],"study_design_scores_gemma":[0.0005269079,0.0006340603,0.0005922588,0.0003941514,0.00001515482,0.000003189166,0.000115905,0.0001671365,0.9872268,0.0001304933,0.009890804,0.0003030707],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9932895,0.00052018,0.001130014,0.0006658426,0.0001793245,0.004042109,0.0000421106,0.0001055619,0.00002538576],"genre_scores_gemma":[0.9942787,0.0003155635,0.001173393,0.00008289643,0.0002240995,0.002293414,0.0002411907,0.00004469462,0.001346106],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0346996,"threshold_uncertainty_score":0.884046,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01776973592706278,"score_gpt":0.2469747422575829,"score_spread":0.2292050063305201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}