{"id":"W4378349374","doi":"10.1002/bit.28435","title":"Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools","year":2023,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; National Research Council Canada","funders":"","keywords":"Chinese hamster ovary cell; Ectodomain; Endoplasmic reticulum; Biology; Monoclonal antibody; Receptor; Cell biology; Transfection; Recombinant DNA; Antibody; Transmembrane protein; G protein-coupled receptor; Membrane protein; Cell culture; Molecular biology; Biochemistry; Gene; Immunology; Genetics; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008575325,0.0007139253,0.0007999639,0.0004591105,0.000279566,0.0009837773,0.0005361563,0.0004504611,0.002464876],"category_scores_gemma":[0.0006985212,0.0002726759,0.0005521725,0.000391362,0.000314441,0.0004713415,0.001029937,0.0007474319,0.002346519],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003643147,"about_ca_system_score_gemma":0.0002624394,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000391664,"about_ca_topic_score_gemma":0.0006804629,"domain_scores_codex":[0.9992325,0.0001226615,0.00008633692,0.0001616072,0.0002801404,0.0001166351],"domain_scores_gemma":[0.9994954,0.000106659,0.0001197378,0.0001033395,0.0001122399,0.00006255994],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004270102,0.00002684135,0.0001699459,0.0000464421,0.00000727595,0.00004848799,0.00002850152,0.00003268058,0.9977769,0.00008054146,0.00007906127,0.001660589],"study_design_scores_gemma":[0.000009783848,0.0001798217,0.001471284,0.0000132724,0.00002361667,0.0001659454,0.00002527754,0.0006154206,0.9912552,0.00004020171,0.006191236,0.000008936946],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9321659,0.002083717,0.0560558,0.0003425397,0.0001301869,0.0005503345,0.001631117,0.0009096985,0.006130728],"genre_scores_gemma":[0.9198679,0.002735027,0.05609068,0.0003132288,0.00006952318,0.000804878,0.005666871,0.0007660043,0.01368592],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002464876,"threshold_uncertainty_score":0.008245885,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005881721406110594,"score_gpt":0.2311326202981064,"score_spread":0.2252508988919958,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}