{"id":"W4378349374","doi":"10.1002/bit.28435","title":"Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools","year":2023,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; National Research Council Canada","funders":"","keywords":"Chinese hamster ovary cell; Ectodomain; Endoplasmic reticulum; Biology; Monoclonal antibody; Receptor; Cell biology; Transfection; Recombinant DNA; Antibody; Transmembrane protein; G protein-coupled receptor; Membrane protein; Cell culture; Molecular biology; Biochemistry; Gene; Immunology; Genetics; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001512738,0.000148064,0.0001759895,0.0001384738,0.0001620931,0.00001163122,0.0001554458,0.0002156688,0.000002810574],"category_scores_gemma":[0.0002204908,0.0001075509,0.00004486909,0.0004877021,0.0001009234,0.0000115312,0.000223514,0.0001197095,5.903016e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008335068,"about_ca_system_score_gemma":0.00002568937,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003814516,"about_ca_topic_score_gemma":0.000006447155,"domain_scores_codex":[0.9990629,0.00003316285,0.0002181745,0.000354028,0.00009730249,0.0002344176],"domain_scores_gemma":[0.9994382,0.0000117322,0.0001175723,0.0003007018,0.00008503475,0.00004678291],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009512039,0.00007248388,0.006019794,0.0002368398,0.00002549001,8.59682e-7,0.00004419518,0.002917877,0.9898311,0.00001603148,0.00009416686,0.0006460142],"study_design_scores_gemma":[0.0002223259,0.0001905036,0.01547514,0.0001743811,0.00001232567,0.00001545385,0.00008801462,0.0002673006,0.9830759,0.00002267654,0.0003234074,0.0001325809],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979303,0.0003560051,0.0008798508,0.0001171296,0.00009985649,0.0004920126,0.0000173956,0.00009947456,0.000007973001],"genre_scores_gemma":[0.9992718,0.0001698998,0.0002314048,0.000004836226,0.00006276042,0.0001394235,0.00001140059,0.00001769083,0.0000907771],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009455343,"threshold_uncertainty_score":0.4385798,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005881721406110594,"score_gpt":0.2311326202981064,"score_spread":0.2252508988919958,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}