{"id":"W4378715049","doi":"10.1186/s12864-023-09377-3","title":"ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells","year":2023,"lang":"en","type":"article","venue":"BMC Genomics","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Precursory Research for Embryonic Science and Technology; Japan Society for the Promotion of Science; Mochida Memorial Foundation for Medical and Pharmaceutical Research; University of Tokyo; Institute of Genetics; Japan Science and Technology Agency; Kato Memorial Bioscience Foundation; Uehara Memorial Foundation; Naito Foundation; Kanae Foundation for the Promotion of Medical Science; Sumitomo Foundation; Takeda Science Foundation","keywords":"CRISPR; Biology; Endogeny; Ploidy; Gene; DNA microarray; Genome editing; Genetics; Cell biology; DNA; Computational biology; Gene expression; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005954544,0.000370359,0.0003726642,0.0002384862,0.0001380302,0.0004593895,0.0002637096,0.0005663885,0.001191491],"category_scores_gemma":[0.0006454154,0.0001646652,0.0003025523,0.0002247637,0.0002391408,0.0003949147,0.0002238859,0.0003987555,0.0004548658],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000348983,"about_ca_system_score_gemma":0.0002531325,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006145885,"about_ca_topic_score_gemma":0.001332848,"domain_scores_codex":[0.9993194,0.00008344498,0.00009909205,0.000162259,0.0002910733,0.00004477978],"domain_scores_gemma":[0.9994148,0.0001534964,0.0001810338,0.00007748498,0.0001127358,0.00006043965],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003806789,0.000006198773,0.0001504805,0.00006525128,0.000005760956,0.00002140963,0.00001226132,0.00004968257,0.9983424,0.00002915186,0.00001633848,0.001262998],"study_design_scores_gemma":[0.000003079871,0.0001706516,0.001303321,0.000008244914,0.00001494333,0.00008445046,0.0000144648,0.0003048711,0.9963993,0.00001917175,0.001673328,0.000004112359],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9624537,0.007025178,0.02645841,0.0001736712,0.000132201,0.00009180144,0.0008036782,0.000339553,0.002521872],"genre_scores_gemma":[0.975387,0.002200963,0.01592032,0.000108159,0.00001258257,0.0000815442,0.001475021,0.00008223597,0.004732161],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001191491,"threshold_uncertainty_score":0.003985941,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0213372227915898,"score_gpt":0.2895664282340374,"score_spread":0.2682292054424476,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}