{"id":"W4379055582","doi":"10.3390/fermentation9060534","title":"Production of Glyoxylate from Glucose in Engineered Escherichia coli","year":2023,"lang":"en","type":"article","venue":"Fermentation","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; National Brain Research Centre; Yale University","keywords":"Glyoxylate cycle; Malate synthase; Isocitrate lyase; Escherichia coli; Biochemistry; Citrate synthase; Biology; Metabolic engineering; Glyoxysome; Chemistry; Enzyme; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001010144,0.00006170893,0.00006863772,0.00006421147,0.00001192319,0.000003998427,0.00003698772,0.00005192847,0.000006966761],"category_scores_gemma":[0.00004923107,0.00006541146,0.00002132714,0.0002453905,0.00001080113,0.000004176794,0.00001444818,0.00003235959,0.00001206321],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000128569,"about_ca_system_score_gemma":0.00001717398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006753618,"about_ca_topic_score_gemma":0.00001397267,"domain_scores_codex":[0.999532,0.00001910401,0.0001377291,0.0001704855,0.00005467195,0.00008603898],"domain_scores_gemma":[0.999802,9.283809e-7,0.00003878991,0.0001177433,0.00002634016,0.00001423063],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000404013,0.00002212245,0.0007222427,0.00001308836,0.00001113536,2.430856e-7,0.0001584578,0.002758408,0.9943427,0.000006597557,0.0003248207,0.00159982],"study_design_scores_gemma":[0.000226961,0.00003506357,0.114965,0.00001030302,0.000005661578,3.663336e-7,0.00006007623,0.0001742324,0.8835518,0.00002688896,0.00088289,0.00006080535],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9988006,0.00009467194,0.0002255625,0.00008287192,0.0006103726,0.0001324546,0.000009839179,0.00002407707,0.00001954336],"genre_scores_gemma":[0.9980523,0.0001743925,0.0005893767,0.000009673856,0.0002300034,0.00001526101,0.000533914,0.000009763521,0.0003853143],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1142427,"threshold_uncertainty_score":0.2667402,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008111619450324214,"score_gpt":0.2317011901428276,"score_spread":0.2235895706925033,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}