{"id":"W4379376951","doi":"10.1002/anie.202303319","title":"A Pro‐Fluorescent Ubiquitin‐Based Probe to Monitor Cysteine‐Based E3 Ligase Activity","year":2023,"lang":"en","type":"article","venue":"Angewandte Chemie International Edition","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"H2020 Marie Skłodowska-Curie Actions; Leibniz-Gemeinschaft; Leids Universitair Medisch Centrum; European Commission; Horizon 2020 Framework Programme; Max-Planck-Gesellschaft; Deutsche Forschungsgemeinschaft; Gottfried Wilhelm Leibniz Universität Hannover; European Federation of Pharmaceutical Industries and Associations; Nederlandse Organisatie voor Wetenschappelijk Onderzoek; McGill University","keywords":"Ubiquitin; Ubiquitin ligase; DNA ligase; Chemistry; Cysteine; Small molecule; Biochemistry; Target protein; Cell biology; Enzyme; Biology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000309515,0.0004835009,0.0002230703,0.0003383616,0.000196369,0.0002531605,0.0005229847,0.0008051865,0.00152008],"category_scores_gemma":[0.0001740262,0.0001723611,0.0001960061,0.0003184862,0.0002914687,0.0003295967,0.0003110692,0.0007349807,0.0004307757],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003650083,"about_ca_system_score_gemma":0.0002585045,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003713495,"about_ca_topic_score_gemma":0.0005600984,"domain_scores_codex":[0.9998119,0.00003696436,0.000006963305,0.000056182,0.00004726764,0.00004074053],"domain_scores_gemma":[0.999903,0.00003057767,0.00002064664,0.00001261956,0.0000130385,0.00002021669],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004208989,0.00001987348,0.00008348197,0.00004508366,0.000003923758,0.00002433682,0.00001000293,0.00006712302,0.9974814,0.000306135,0.0001254611,0.001791173],"study_design_scores_gemma":[0.00000587661,0.0001284728,0.0003517273,0.000002752537,0.000006022491,0.00009847306,0.000007833908,0.0009142282,0.9967766,0.00005064513,0.001652645,0.00000474675],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8130153,0.005678912,0.1701289,0.0004936136,0.000120195,0.0001353768,0.0009364457,0.0009999023,0.008491307],"genre_scores_gemma":[0.9247842,0.002183635,0.0648959,0.0002698212,0.00003031528,0.0001778418,0.0005355214,0.00004691402,0.007075819],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00152008,"threshold_uncertainty_score":0.005085111,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02443011057455743,"score_gpt":0.2888358408753892,"score_spread":0.2644057303008318,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}