{"id":"W4379508237","doi":"10.1021/acssynbio.3c00114","title":"Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministerie van Onderwijs, Cultuur en Wetenschap; Radboud Universiteit; Alfred P. Sloan Foundation; Université de Sherbrooke; National Science Foundation","keywords":"Synthetic biology; Cell-free protein synthesis; Escherichia coli; Lysis; Bacteria; Ribonuclease; Computational biology; Biology; Cell-free system; Biochemistry; In vitro; Protein biosynthesis; Chemistry; Genetics; RNA; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004362961,0.0005327041,0.0004807722,0.000291219,0.0002682247,0.0004038577,0.0004190567,0.000412024,0.001058053],"category_scores_gemma":[0.0003515971,0.0002666855,0.0003389391,0.0002824148,0.000275941,0.0003340323,0.0004011912,0.0009296268,0.001070661],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003390769,"about_ca_system_score_gemma":0.0003124996,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000353832,"about_ca_topic_score_gemma":0.0006484641,"domain_scores_codex":[0.9996622,0.00004938587,0.00004028655,0.00008260384,0.0001314278,0.00003413123],"domain_scores_gemma":[0.9998491,0.00003562464,0.00003457462,0.0000259158,0.00002926518,0.00002544995],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002083619,0.000009655664,0.00005040006,0.00003772501,0.000002547448,0.00002263569,0.00001331205,0.00009220403,0.9982517,0.0002288813,0.00007404847,0.001196058],"study_design_scores_gemma":[0.000004935478,0.00006851341,0.0003223025,0.000004974424,0.000008156495,0.00009653733,0.00001197843,0.001213629,0.9917846,0.0000690181,0.006407272,0.000008077938],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6736092,0.001292841,0.3097665,0.0004868986,0.0002388713,0.000566008,0.004429237,0.002422554,0.007187929],"genre_scores_gemma":[0.7137368,0.001437176,0.260012,0.0002114966,0.0000376644,0.0006453363,0.01188465,0.0007716144,0.01126323],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001058053,"threshold_uncertainty_score":0.003539503,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009534644669830115,"score_gpt":0.2373156088775722,"score_spread":0.227780964207742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}