{"id":"W4379508237","doi":"10.1021/acssynbio.3c00114","title":"Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministerie van Onderwijs, Cultuur en Wetenschap; Radboud Universiteit; Alfred P. Sloan Foundation; Université de Sherbrooke; National Science Foundation","keywords":"Synthetic biology; Cell-free protein synthesis; Escherichia coli; Lysis; Bacteria; Ribonuclease; Computational biology; Biology; Cell-free system; Biochemistry; In vitro; Protein biosynthesis; Chemistry; Genetics; RNA; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001779631,0.0003425451,0.0003409765,0.00007717549,0.0002943504,0.00005993826,0.000615835,0.0004897504,0.0001634257],"category_scores_gemma":[0.0002073778,0.0002913541,0.0002071013,0.0001659868,0.0002582741,0.000006295023,0.0007355142,0.0001526932,0.0004542629],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002546431,"about_ca_system_score_gemma":0.00009218977,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001100044,"about_ca_topic_score_gemma":0.000007364693,"domain_scores_codex":[0.9977248,0.0003052373,0.0003869708,0.0008594785,0.0001325512,0.0005909847],"domain_scores_gemma":[0.9982378,0.00009907294,0.0001728059,0.001204792,0.00007817456,0.0002073837],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001536288,0.00007423997,0.001233754,0.00004442274,0.00004271495,0.00001208504,0.00006658445,0.00004822788,0.9871063,0.00004438494,0.01050322,0.0006704959],"study_design_scores_gemma":[0.0009716934,0.0005247413,0.003079027,0.0001206156,0.00005853487,0.0000303066,0.0003020916,0.000227004,0.9733345,0.0003558447,0.02048276,0.0005129091],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946079,0.000822574,0.0005246876,0.0001978817,0.001180361,0.000266794,0.0003218359,0.0002148317,0.001863103],"genre_scores_gemma":[0.9977512,0.0001748302,0.0003928236,0.0001976545,0.0004359125,0.0001625794,0.0004550528,0.00006368926,0.0003662909],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01377177,"threshold_uncertainty_score":0.9999539,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009534644669830115,"score_gpt":0.2373156088775722,"score_spread":0.227780964207742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}