{"id":"W4379988652","doi":"10.3390/iecb2023-14563","title":"Directed Evolution of a Genetically Encoded Bioluminescent Ca2+ Sensor","year":2023,"lang":"en","type":"article","venue":"","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"University of Alberta","keywords":"Bioluminescence; Luciferase; Fluorescence; Phototoxicity; Calcium imaging; In vivo; Biology; Biophysics; Cell biology; Intracellular; Calcium; In vitro; Chemistry; Cell culture; Biochemistry; Genetics; Transfection; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005586892,0.0006856312,0.0005386139,0.0004755833,0.000174801,0.0006754411,0.0007822627,0.0007658996,0.0004465737],"category_scores_gemma":[0.001016209,0.0002917915,0.0003764781,0.0007707499,0.0003278142,0.0002583042,0.0005511062,0.001243684,0.0003274838],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005113656,"about_ca_system_score_gemma":0.0003800836,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001311476,"about_ca_topic_score_gemma":0.001653429,"domain_scores_codex":[0.9995877,0.00007674554,0.00005922044,0.00008241789,0.000125098,0.00006876888],"domain_scores_gemma":[0.9996879,0.00008725862,0.00007970373,0.0000336495,0.0000690148,0.00004242207],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005823008,0.00005751173,0.0003666258,0.00005642712,0.0000254566,0.0001281336,0.00004327312,0.001237803,0.9932724,0.0003625795,0.00008469954,0.004306921],"study_design_scores_gemma":[0.00004476196,0.0002025101,0.002394373,0.00002183218,0.00006174464,0.0003700863,0.00005349029,0.01336971,0.977091,0.000107032,0.006252624,0.00003083129],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9124113,0.001209257,0.08153459,0.0003182665,0.0001253894,0.0003255668,0.0009632438,0.0005302444,0.002582113],"genre_scores_gemma":[0.9105436,0.001249404,0.0815023,0.0002066399,0.00001483402,0.0002239077,0.0019459,0.0002592205,0.004054215],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001311476,"threshold_uncertainty_score":0.00371021,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01402318878258581,"score_gpt":0.2736379119694071,"score_spread":0.2596147231868213,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}