{"id":"W4380480141","doi":"10.1016/j.xpro.2023.102351","title":"Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture","year":2023,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Cancer Research and Treatments","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; University of Calgary","funders":"New Harvest","keywords":"Cloning (programming); Growth factor; Workflow; Protein expression; Cell growth; Cell culture; Computational biology; Biotechnology; Cell biology; Computer science; Biology; Chemistry; Biochemistry; Genetics; Gene; Database","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005660025,0.00008340054,0.00008300164,0.00003351531,0.00003517985,0.00001856824,0.00004196552,0.00008683859,0.000001083689],"category_scores_gemma":[0.00003876082,0.0000575244,0.00002244948,0.00008336439,0.00003367261,0.000009006648,0.00004229157,0.00004032421,2.90326e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009250439,"about_ca_system_score_gemma":0.00002176626,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008964095,"about_ca_topic_score_gemma":0.00004931695,"domain_scores_codex":[0.9994726,0.00003135295,0.00008536726,0.0002143866,0.0000712814,0.0001249801],"domain_scores_gemma":[0.9997479,0.000007143416,0.00004805952,0.00009354946,0.00005830493,0.00004498884],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000195905,0.00005524013,0.00674831,0.0001575097,0.000008504162,8.52974e-7,0.0001688211,3.567309e-7,0.9922251,0.00001404652,0.0003025467,0.0001228085],"study_design_scores_gemma":[0.001033442,0.0004383855,0.005973266,0.00005205899,0.000002328956,1.666963e-7,0.0001829369,0.000004317123,0.9892151,0.0001191304,0.002912908,0.00006600868],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9629818,0.00005740732,0.00006057365,0.00001371934,0.000002669431,0.03670204,0.0001544499,0.000005546641,0.00002175855],"genre_scores_gemma":[0.9475942,0.00002746394,0.000678614,0.000002847182,0.00001860127,0.05092771,0.0003002255,0.000009497726,0.0004408493],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01538765,"threshold_uncertainty_score":0.2345777,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02518261928905732,"score_gpt":0.3037223119133612,"score_spread":0.2785396926243039,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}