{"id":"W4381166430","doi":"10.3390/ijms241210266","title":"A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells","year":2023,"lang":"en","type":"article","venue":"International Journal of Molecular Sciences","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"Biotechnology and Biological Sciences Research Council; Medical Research Council; Newcastle University; Company of Biologists; National Centre for the Replacement, Refinement and Reduction of Animals in Research; Cystic Fibrosis Trust; Cystic Fibrosis Foundation","keywords":"Induced pluripotent stem cell; Genome editing; CRISPR; Transcription activator-like effector nuclease; Zinc finger nuclease; Cas9; Biology; Computational biology; Cell sorting; Mutation; ΔF508; Gene; Genetics; Embryonic stem cell; Cell","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003506012,0.000417429,0.0003778215,0.0002893818,0.0001895621,0.0004437294,0.0003500543,0.0005624785,0.001096571],"category_scores_gemma":[0.0002107086,0.0002152031,0.0003113214,0.0002380664,0.0002199572,0.0001910272,0.0003504296,0.0008158731,0.0008256977],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002376724,"about_ca_system_score_gemma":0.0003411475,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004332621,"about_ca_topic_score_gemma":0.0008918271,"domain_scores_codex":[0.9996272,0.00003579568,0.00003921209,0.00009794383,0.0001691569,0.00003063572],"domain_scores_gemma":[0.9999138,0.00001705493,0.00001936385,0.0000164072,0.00001917245,0.00001426048],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001657785,0.000009817378,0.00003943279,0.00003212771,0.000003493514,0.00003297852,0.000008295571,0.0002505219,0.9969228,0.000178176,0.00006498535,0.00244096],"study_design_scores_gemma":[0.00001005934,0.000102198,0.0004769652,0.000004817895,0.00001572455,0.0002474873,0.00000618555,0.00211299,0.9890325,0.00007153749,0.007905752,0.000013738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4685386,0.003301844,0.5142127,0.0003752204,0.0004263216,0.0008159347,0.002578539,0.002057534,0.007693379],"genre_scores_gemma":[0.6883883,0.002239914,0.2975994,0.0001875746,0.00003330813,0.0005439571,0.002669383,0.0002381927,0.008099975],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001096571,"threshold_uncertainty_score":0.003668427,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01457648012166906,"score_gpt":0.2956252247852787,"score_spread":0.2810487446636096,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}