{"id":"W4381166430","doi":"10.3390/ijms241210266","title":"A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells","year":2023,"lang":"en","type":"article","venue":"International Journal of Molecular Sciences","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"Biotechnology and Biological Sciences Research Council; Medical Research Council; Newcastle University; Company of Biologists; National Centre for the Replacement, Refinement and Reduction of Animals in Research; Cystic Fibrosis Trust; Cystic Fibrosis Foundation","keywords":"Induced pluripotent stem cell; Genome editing; CRISPR; Transcription activator-like effector nuclease; Zinc finger nuclease; Cas9; Biology; Computational biology; Cell sorting; Mutation; ΔF508; Gene; Genetics; Embryonic stem cell; Cell","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005153221,0.00007363276,0.00009717882,0.0001458503,0.00004529104,0.0000299208,0.0002913078,0.00003453053,0.000001481177],"category_scores_gemma":[0.00001690951,0.00006460398,0.0001014974,0.00008668624,0.00008598632,0.000002764069,0.0000562181,0.00003664453,1.282082e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007138046,"about_ca_system_score_gemma":0.0000550294,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000001355406,"about_ca_topic_score_gemma":9.794078e-8,"domain_scores_codex":[0.99915,0.00001828293,0.0002452229,0.000144195,0.0003250659,0.00011717],"domain_scores_gemma":[0.9995006,0.00001568219,0.0001702896,0.00006136994,0.0002034961,0.00004860518],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001296985,0.00002524814,0.00003217809,0.00001216778,0.00002076119,0.00000412462,0.00003335664,0.2848925,0.7143505,0.00001082742,0.00005531025,0.0005500235],"study_design_scores_gemma":[0.0005953339,0.0003060963,0.00008983551,0.00001695933,0.00001186052,0.00001329027,0.0001820853,0.04167899,0.9568934,0.000009176082,0.0001389196,0.00006407806],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7885559,0.0006324016,0.210358,0.00006668022,0.0002256999,0.00007199126,0.000008850464,0.000002661659,0.00007778958],"genre_scores_gemma":[0.9853451,0.00003617375,0.01442346,0.00003646524,0.0001247799,0.000003638649,0.00000686316,0.000006757366,0.00001674315],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2432135,"threshold_uncertainty_score":0.2634474,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01457648012166906,"score_gpt":0.2956252247852787,"score_spread":0.2810487446636096,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}