{"id":"W4381309216","doi":"10.1002/biot.202300051","title":"Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities","year":2023,"lang":"en","type":"article","venue":"Biotechnology Journal","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Polytechnique Montréal; McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Transfection; Genetic enhancement; Cell; Titer; Cell culture; Biology; HEK 293 cells; Transgene; Plasmid; Virus; Molecular biology; Cell biology; Virology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006776949,0.0006717604,0.0008258653,0.0003939179,0.0002263036,0.001013568,0.0005572705,0.0007729594,0.002020386],"category_scores_gemma":[0.000340661,0.0002982651,0.0006236765,0.000343614,0.0003499118,0.0005758563,0.0004793861,0.002050515,0.001729451],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004804607,"about_ca_system_score_gemma":0.0002884178,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000736888,"about_ca_topic_score_gemma":0.0007761861,"domain_scores_codex":[0.9993502,0.00009611517,0.0001004456,0.0001313576,0.0002552891,0.00006651839],"domain_scores_gemma":[0.9997548,0.00006096591,0.00004654215,0.00003771773,0.00006268691,0.00003724029],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001730625,0.00002199686,0.00004987649,0.00003357973,0.000003946596,0.0000286542,0.00002035887,0.00005826188,0.9989385,0.0001151259,0.00004106016,0.0006713463],"study_design_scores_gemma":[0.000007247532,0.000185003,0.000515936,0.0000070637,0.00001705468,0.0001361406,0.000022377,0.001776171,0.9940296,0.00004824227,0.00324609,0.00000902283],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8555168,0.002626701,0.1295269,0.0004328646,0.000471316,0.0004753436,0.002831893,0.000998349,0.007119922],"genre_scores_gemma":[0.8752374,0.001938324,0.1015631,0.0002446125,0.0000593412,0.0002859998,0.005565844,0.0002623383,0.01484297],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002020386,"threshold_uncertainty_score":0.006758869,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01199742779657519,"score_gpt":0.2506283613015268,"score_spread":0.2386309335049516,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}