{"id":"W4381309216","doi":"10.1002/biot.202300051","title":"Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities","year":2023,"lang":"en","type":"article","venue":"Biotechnology Journal","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Polytechnique Montréal; McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Transfection; Genetic enhancement; Cell; Titer; Cell culture; Biology; HEK 293 cells; Transgene; Plasmid; Virus; Molecular biology; Cell biology; Virology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004644705,0.0001425218,0.0002318333,0.0002287859,0.0001311521,0.000006561607,0.0001908195,0.0005617257,0.00006259732],"category_scores_gemma":[0.00004896513,0.0001354416,0.0001217715,0.0003612915,0.0002168477,0.000006221022,0.00006951176,0.0002983516,0.00001750036],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006930573,"about_ca_system_score_gemma":0.00007983785,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001496318,"about_ca_topic_score_gemma":0.00006604305,"domain_scores_codex":[0.9987323,0.0001624965,0.000320256,0.0002552948,0.0002251798,0.0003044829],"domain_scores_gemma":[0.9993042,0.00001044531,0.0002034558,0.0002527854,0.0001812251,0.00004783867],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001366782,0.000102029,0.0003228887,0.00002183054,0.00008265914,0.000004561831,0.00003186065,0.0000552869,0.9824334,0.000003247798,0.01498161,0.0005937868],"study_design_scores_gemma":[0.0008526386,0.003098245,0.0007391135,0.00001659384,0.00002288875,0.00002728254,0.00006238874,0.00001962873,0.9926025,0.00004170386,0.002398336,0.0001186497],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972526,0.0009275115,0.00003142678,0.0008609268,0.0006454345,0.0001925212,0.00002479456,0.0000411857,0.00002363013],"genre_scores_gemma":[0.9949114,0.001697073,0.00003315681,0.00001757941,0.0001090878,0.000004956296,0.00006042389,0.00002922953,0.003137144],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01258327,"threshold_uncertainty_score":0.5523148,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01199742779657519,"score_gpt":0.2506283613015268,"score_spread":0.2386309335049516,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}