{"id":"W4381489126","doi":"10.17504/protocols.io.261ged6xyv47/v1","title":"Converting ssDNA oligos to dsDNA with T4 DNA polymerase v1","year":2023,"lang":"en","type":"preprint","venue":"","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Oligonucleotide; Polymerase; Chemistry; Molecular biology; Computational biology; DNA; Biology; Physics; Computer science; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004477403,0.001152794,0.0007186315,0.0008875258,0.0005668349,0.0007912673,0.0007125146,0.000691709,0.01373406],"category_scores_gemma":[0.0008191792,0.0009358632,0.0005827711,0.0008576628,0.0004054188,0.0004405214,0.0007001437,0.002441268,0.01804592],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003330215,"about_ca_system_score_gemma":0.0005723892,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005642462,"about_ca_topic_score_gemma":0.0009119914,"domain_scores_codex":[0.9994339,0.00006380535,0.00006340905,0.0001957108,0.0001536459,0.00008939535],"domain_scores_gemma":[0.9997243,0.0000576294,0.0000286408,0.00008398089,0.00006694072,0.00003850914],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009712108,0.00003797832,0.0001163336,0.0002471908,0.00001487762,0.0001154121,0.00008034875,0.0001952617,0.9792083,0.00202925,0.00336306,0.014495],"study_design_scores_gemma":[0.00001971263,0.00006258851,0.0002944447,0.00001815833,0.00001795313,0.0002515027,0.00002024279,0.0005797576,0.9088902,0.0009747435,0.08884765,0.00002299875],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1184708,0.002371014,0.7985221,0.0006005358,0.001912974,0.003331736,0.01609537,0.01528721,0.04340826],"genre_scores_gemma":[0.2132011,0.004367196,0.5734302,0.000899318,0.0002500199,0.003312485,0.07227592,0.007975404,0.1242884],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01373406,"threshold_uncertainty_score":0.04594505,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01736892036933469,"score_gpt":0.286420228127682,"score_spread":0.2690513077583473,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}