{"id":"W4381620548","doi":"10.1016/j.ab.2023.115182","title":"Quantifying metal-binding specificity of CcNikZ-II from Clostridium carboxidivorans in the presence of competing metal ions","year":2023,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Trace Elements in Health","field":"Nursing","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; U.S. Department of Energy; Ministero dello Sviluppo Economico; Connaught Fund; Lawrence Livermore National Laboratory; University of Toronto","keywords":"Affinities; Chemistry; Metal; Metalloprotein; Metal ions in aqueous solution; Binding affinities; Binding site; Cooperative binding; Plasma protein binding; Biochemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00112462,0.0001951738,0.0004626334,0.0001195473,0.000149084,0.00001889801,0.000561643,0.000145703,0.00006522371],"category_scores_gemma":[0.001090169,0.0001644937,0.0001890512,0.001206113,0.0002761157,0.00006606992,0.0001636707,0.000489087,0.0000109368],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007599296,"about_ca_system_score_gemma":0.00005941632,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008757141,"about_ca_topic_score_gemma":0.0000753375,"domain_scores_codex":[0.9974786,0.0001750724,0.0008150961,0.0004236406,0.0006331285,0.0004744686],"domain_scores_gemma":[0.9975397,0.001461975,0.0002577962,0.0005818215,0.00007553231,0.00008319276],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001264465,0.0004109297,0.05640917,0.0003798159,0.0001403328,0.0000236039,0.001450825,0.000122445,0.9390774,0.0007628711,0.0007438114,0.0003523651],"study_design_scores_gemma":[0.0004113038,0.00006603259,0.00739407,0.0002566758,0.0001668925,0.000002481614,0.004294155,0.004056968,0.982827,0.0001566077,0.0001839254,0.000183855],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965467,0.0001673056,0.00001627501,0.001388593,0.0001745612,0.0002018133,0.0001290992,0.00004643111,0.00132921],"genre_scores_gemma":[0.9992533,0.0000175824,0.0004058085,0.00003102479,0.0001443315,0.000007233861,0.00008264699,0.00001835094,0.00003970333],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0490151,"threshold_uncertainty_score":0.6707859,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07392645718628839,"score_gpt":0.3517473305579964,"score_spread":0.277820873371708,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}