{"id":"W4381735249","doi":"10.3791/65463","title":"Methods for Electroporation and Transformation Confirmation in <em>Limosilactobacillus reuteri</em> DSM20016","year":2023,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Microbial Inactivation Methods","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Lactobacillus reuteri; Transformation (genetics); Biology; Plasmid; Computational biology; Computer science; Genetics; Lactobacillus; Bacteria; DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003329432,0.001801854,0.001386986,0.001899351,0.001237379,0.001151191,0.002439452,0.001054878,0.00878619],"category_scores_gemma":[0.002783505,0.001114527,0.001127226,0.00163016,0.001211345,0.0008464922,0.001710365,0.002997529,0.01772007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005633914,"about_ca_system_score_gemma":0.001161599,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001081369,"about_ca_topic_score_gemma":0.002474396,"domain_scores_codex":[0.9951968,0.001388195,0.0008971529,0.0009037673,0.00117871,0.0004355301],"domain_scores_gemma":[0.9978688,0.0005567347,0.000340354,0.0007687072,0.0003246367,0.0001408039],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002585136,0.0001839948,0.0004292392,0.0005256466,0.00003120193,0.0003240815,0.0002979543,0.0002537172,0.9637765,0.002298939,0.004266352,0.02735376],"study_design_scores_gemma":[0.00006829149,0.0003686385,0.002002798,0.0001526507,0.00007651691,0.0007538337,0.0001474349,0.001522773,0.8629447,0.0007679944,0.1311226,0.00007175216],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.04473865,0.002607042,0.909099,0.001232601,0.0008624862,0.007113717,0.008228798,0.007074801,0.01904288],"genre_scores_gemma":[0.08648139,0.005380637,0.8223364,0.0007200743,0.000169601,0.01274716,0.03236984,0.003651859,0.03614308],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00878619,"threshold_uncertainty_score":0.02939278,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03933947410622474,"score_gpt":0.4526400157387341,"score_spread":0.4133005416325093,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}