{"id":"W4383032096","doi":"10.1016/j.xpro.2023.102409","title":"Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry","year":2023,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health; Royal Bank of Canada","keywords":"Flow cytometry; Cell; RNA; Biology; Isolation (microbiology); Single-cell analysis; Computational biology; Cell biology; Molecular biology; Bioinformatics; Genetics; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004828273,0.0002654913,0.0002805057,0.000100967,0.0002087953,0.00009539627,0.0001878873,0.0002408105,0.00001173186],"category_scores_gemma":[0.00009532912,0.000257968,0.0001545616,0.0001658284,0.00005581956,0.00001373433,0.00006581432,0.00009271096,0.000004068223],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006488076,"about_ca_system_score_gemma":0.0001405746,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008172863,"about_ca_topic_score_gemma":0.000002419466,"domain_scores_codex":[0.9984289,0.00005327807,0.0004064826,0.000502298,0.000116444,0.0004925794],"domain_scores_gemma":[0.9991812,0.00005376698,0.0001665802,0.000332601,0.0001725303,0.0000933269],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001683023,0.0001367733,0.00004874877,0.00103475,0.00002349678,7.935442e-7,0.0001716401,0.0004426155,0.9946315,0.000004165804,0.0005567693,0.001265681],"study_design_scores_gemma":[0.009349684,0.002006069,0.000009778526,0.00006153321,0.00001154224,0.000001939592,0.0001125541,0.007747861,0.9565343,0.00012158,0.02370576,0.0003374291],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.04798559,0.00001023432,0.07919966,0.00003987861,0.00005898658,0.8720764,0.0001765599,0.0001074755,0.000345231],"genre_scores_gemma":[0.01688716,0.000001296138,0.03847842,0.00005789312,0.0001628368,0.9425455,0.0002011109,0.00008495699,0.001580798],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.07046915,"threshold_uncertainty_score":0.9999872,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05202782259551355,"score_gpt":0.3105929304865125,"score_spread":0.258565107890999,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}