{"id":"W4383032096","doi":"10.1016/j.xpro.2023.102409","title":"Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry","year":2023,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health; Royal Bank of Canada","keywords":"Flow cytometry; Cell; RNA; Biology; Isolation (microbiology); Single-cell analysis; Computational biology; Cell biology; Molecular biology; Bioinformatics; Genetics; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002273273,0.002237504,0.001587821,0.002755106,0.002031347,0.00124359,0.001851376,0.001326898,0.03181912],"category_scores_gemma":[0.001260905,0.001515749,0.00137477,0.001574453,0.0007710819,0.0007550091,0.001257175,0.004385152,0.02634192],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007536138,"about_ca_system_score_gemma":0.001887593,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001524624,"about_ca_topic_score_gemma":0.005519697,"domain_scores_codex":[0.9978437,0.0003112683,0.0002529594,0.0006631018,0.0006701918,0.000258678],"domain_scores_gemma":[0.9989927,0.0001682287,0.00007702144,0.0002794736,0.0003666303,0.0001158328],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0004125104,0.0002089482,0.0005843785,0.0008343466,0.00008779595,0.0002893681,0.0002055601,0.0008058834,0.9446957,0.002377456,0.02390568,0.02559225],"study_design_scores_gemma":[0.0002865148,0.0005844767,0.004688758,0.0002813105,0.0002202812,0.001286999,0.00008912286,0.004041036,0.5426313,0.0032674,0.442412,0.0002108815],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.03343206,0.004763938,0.8338184,0.0007668406,0.001556153,0.01054228,0.06700145,0.0204408,0.02767804],"genre_scores_gemma":[0.04225711,0.004462309,0.7334806,0.001531456,0.0002867194,0.0322039,0.1188147,0.005273399,0.0616899],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.03181912,"threshold_uncertainty_score":0.1064455,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05202782259551355,"score_gpt":0.3105929304865125,"score_spread":0.258565107890999,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}