{"id":"W4383058472","doi":"10.1101/pdb.prot108162","title":"Proximity Ligation Assay (PLA) for Fillets of<i>Drosophila</i>Larvae","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Molecular Junctions and Nanostructures","field":"Engineering","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Proximity ligation assay; Förster resonance energy transfer; Oligonucleotide; Primary and secondary antibodies; In vivo; Immunoprecipitation; Epitope; Molecular biology; Rolling circle replication; In vitro; Biology; Protein–protein interaction; Biophysics; Antibody; Cell biology; Fluorescence; Chemistry; Biochemistry; Gene; Receptor; Genetics; DNA replication","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001021132,0.00171737,0.001120808,0.00142525,0.0009285623,0.0006521701,0.001346057,0.001080247,0.00693207],"category_scores_gemma":[0.0008189818,0.001267433,0.001425078,0.0007165806,0.000487756,0.0006951884,0.0008912269,0.002341026,0.007593714],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007026406,"about_ca_system_score_gemma":0.0006322854,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001184877,"about_ca_topic_score_gemma":0.004246278,"domain_scores_codex":[0.9981372,0.0003268847,0.0002247835,0.0005222211,0.0006128587,0.000175942],"domain_scores_gemma":[0.9993686,0.0001736994,0.0001452746,0.0001009711,0.0001446053,0.00006690573],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00004912231,0.00002820612,0.0001865672,0.0001339832,0.00001450688,0.00008893289,0.00004190381,0.00005806633,0.9940845,0.0002132275,0.000605174,0.004495786],"study_design_scores_gemma":[0.0000123759,0.0001596648,0.001702022,0.00004879827,0.00003686042,0.0002878548,0.00003210219,0.00147162,0.9784572,0.0001182591,0.01764619,0.00002713296],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1543506,0.006220351,0.7886091,0.0008900024,0.0006358453,0.003320562,0.009062658,0.01131104,0.02559984],"genre_scores_gemma":[0.1644555,0.00668531,0.7617761,0.0005549268,0.00008831971,0.005578367,0.01888221,0.001695539,0.04028374],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00693207,"threshold_uncertainty_score":0.02319014,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0172313860462876,"score_gpt":0.251506832007273,"score_spread":0.2342754459609854,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}