{"id":"W4383058472","doi":"10.1101/pdb.prot108162","title":"Proximity Ligation Assay (PLA) for Fillets of<i>Drosophila</i>Larvae","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Molecular Junctions and Nanostructures","field":"Engineering","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Proximity ligation assay; Förster resonance energy transfer; Oligonucleotide; Primary and secondary antibodies; In vivo; Immunoprecipitation; Epitope; Molecular biology; Rolling circle replication; In vitro; Biology; Protein–protein interaction; Biophysics; Antibody; Cell biology; Fluorescence; Chemistry; Biochemistry; Gene; Receptor; Genetics; DNA replication","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002924319,0.000148176,0.0001947206,0.0001303733,0.00008655919,0.0000372611,0.0001524454,0.0001117624,0.00001532718],"category_scores_gemma":[0.0001232422,0.0001527095,0.0001068442,0.0003392383,0.00002363321,0.00009988465,0.00003569403,0.0001118647,0.00002093845],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003788004,"about_ca_system_score_gemma":0.0000279628,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003549012,"about_ca_topic_score_gemma":0.000001738825,"domain_scores_codex":[0.9991026,0.00002069212,0.0002603255,0.0001931164,0.0001709356,0.0002523129],"domain_scores_gemma":[0.9994418,0.00005736379,0.00005814965,0.0003022363,0.0000801128,0.00006034286],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002994388,0.0000250428,0.0005583663,0.0009376038,0.00005883638,0.000004774459,0.00002625641,0.007863451,0.9737734,0.01247234,0.003430279,0.0008197031],"study_design_scores_gemma":[0.0008314747,0.00009870023,0.01467163,0.000216734,0.00001829235,4.431535e-7,0.000002601825,0.0246842,0.8531805,0.0004576346,0.1055531,0.0002846666],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5295485,0.0002052445,0.08929322,0.0004439738,0.001568588,0.3720411,0.0003095517,0.004611217,0.001978544],"genre_scores_gemma":[0.8600712,0.000005036116,0.003310001,0.00002377444,0.0001574864,0.1362644,0.000004463833,0.00007112302,0.00009246701],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3305227,"threshold_uncertainty_score":0.6227311,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0172313860462876,"score_gpt":0.251506832007273,"score_spread":0.2342754459609854,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}