{"id":"W4383500228","doi":"10.1021/acsomega.3c03064","title":"Mapping Peptide–Protein Interactions by Amine-Reactive Cleavable Photoaffinity Reagents","year":2023,"lang":"en","type":"article","venue":"ACS Omega","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; CancerCare Manitoba; Research Manitoba; McGill University","funders":"Fonds de recherche du Québec – Nature et technologies; Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen; Fonds Wetenschappelijk Onderzoek; Bundesministerium für Bildung und Forschung; University of Manitoba","keywords":"Photoaffinity labeling; Chemistry; Peptide; Melittin; Binding site; Combinatorial chemistry; Tandem mass spectrometry; Drug discovery; Small molecule; Plasma protein binding; Target protein; Biochemistry; Mass spectrometry; Chromatography","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003796009,0.0005562195,0.0002385733,0.0003737139,0.0003007324,0.000417894,0.0005308755,0.0005904608,0.001903743],"category_scores_gemma":[0.0004495714,0.0003544882,0.0003402997,0.0002576377,0.0003863808,0.0004047584,0.0003228071,0.001178817,0.001045657],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004629187,"about_ca_system_score_gemma":0.0002784257,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005603397,"about_ca_topic_score_gemma":0.001286349,"domain_scores_codex":[0.9997148,0.00005659673,0.00001504314,0.00008249789,0.00008637579,0.00004470667],"domain_scores_gemma":[0.9996859,0.0001262371,0.00008387298,0.00003754117,0.00003776775,0.00002865344],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001387595,0.000005666966,0.00003134003,0.00002020204,0.000003254705,0.00001890427,0.00000662847,0.00004230575,0.9990216,0.00008702018,0.00002055964,0.0007286521],"study_design_scores_gemma":[0.000003177382,0.00004314463,0.0003085456,0.000002208006,0.000004505632,0.00007304912,0.000007953289,0.0004482925,0.9980901,0.00005128337,0.0009644581,0.000003192901],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7432008,0.004904208,0.2443537,0.0004655255,0.0001061793,0.0002622902,0.000577885,0.0006087391,0.005520748],"genre_scores_gemma":[0.8548197,0.004843189,0.1307935,0.0004491169,0.00004485775,0.0003075735,0.001022835,0.000155002,0.007564298],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001903743,"threshold_uncertainty_score":0.006368637,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01495951122043928,"score_gpt":0.2537744393140098,"score_spread":0.2388149280935705,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}