{"id":"W4383702594","doi":"10.1186/s12934-023-02135-w","title":"Construction and characterization of a novel miniaturized filamentous phagemid for targeted mammalian gene transfer","year":2023,"lang":"en","type":"article","venue":"Microbial Cell Factories","topic":"Cancer Research and Treatments","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Mitacs; Consejo Nacional de Ciencia y Tecnología","keywords":"Biology; Transgene; Plasmid; Phagemid; Gene; Genome; Genetics; DNA; Recombinase; Horizontal gene transfer; Gene silencing; Bacteriophage; Expression cassette; Computational biology; Vector (molecular biology); Escherichia coli; Recombination; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004854891,0.0004082987,0.0003082358,0.000299714,0.0002228362,0.0004708623,0.0004774666,0.0004688898,0.0007947463],"category_scores_gemma":[0.0003980284,0.0001647409,0.0003981203,0.0002176791,0.0002434519,0.0002777798,0.0002663872,0.0005136119,0.0007412904],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006555111,"about_ca_system_score_gemma":0.0004033525,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008800376,"about_ca_topic_score_gemma":0.0008185488,"domain_scores_codex":[0.9998017,0.00001827287,0.00001733578,0.00003913065,0.0000931563,0.00003045647],"domain_scores_gemma":[0.9997723,0.00003590122,0.00005066647,0.00002061935,0.00005165119,0.00006898858],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002044864,0.00002786731,0.0001826349,0.00003756533,0.000004714041,0.00005716567,0.00001709765,0.0001259656,0.9973904,0.0001023389,0.00005447649,0.001979255],"study_design_scores_gemma":[0.00001998066,0.0002777147,0.00305837,0.00001463996,0.00002879823,0.000375253,0.00002051133,0.002113249,0.9829203,0.00005339978,0.0111032,0.00001447638],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8573132,0.001629431,0.1339457,0.0003376993,0.0001405744,0.0008752627,0.002016766,0.0006592589,0.003082171],"genre_scores_gemma":[0.8195665,0.00162932,0.1604399,0.0001597598,0.00004522904,0.0004774044,0.008922734,0.0001953951,0.008563668],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008800376,"threshold_uncertainty_score":0.004756153,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01249413611779187,"score_gpt":0.2416588355923851,"score_spread":0.2291646994745932,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}