{"id":"W4384283659","doi":"10.1016/j.omtm.2023.07.002","title":"Inducible HEK293 AAV packaging cell lines expressing Rep proteins","year":2023,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université Laval; National Research Council Canada","funders":"Fonds de Recherche du Québec - Santé; National Research Council Canada; Natural Sciences and Engineering Research Council of Canada","keywords":"HEK 293 cells; Transfection; Cell culture; Biology; Transduction (biophysics); Plasmid; Titer; Molecular biology; Cell biology; Viral vector; Activator (genetics); Recombinant DNA; Gene; Virus; Virology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00107167,0.0006719009,0.0007571407,0.0005891908,0.000492073,0.00113294,0.001205295,0.00102495,0.008154293],"category_scores_gemma":[0.0008537418,0.0004955754,0.0005892689,0.0007486363,0.000370376,0.0005330917,0.000584551,0.001911091,0.011607],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003581319,"about_ca_system_score_gemma":0.0004443732,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005362731,"about_ca_topic_score_gemma":0.0007943794,"domain_scores_codex":[0.9985653,0.0002062906,0.0002273971,0.0002739487,0.0005794997,0.0001477363],"domain_scores_gemma":[0.999306,0.0001511531,0.00009872096,0.0001759619,0.0002122371,0.00005588122],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008855329,0.00007267007,0.000212391,0.0001708849,0.00001095191,0.0001575617,0.00008207189,0.0001582952,0.9918417,0.0007556665,0.001537346,0.004911878],"study_design_scores_gemma":[0.00004370834,0.0003507808,0.001471459,0.0000320463,0.00005722861,0.001066716,0.00005598539,0.002618925,0.9338419,0.0002867416,0.06013985,0.00003463961],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3863184,0.005561301,0.5069934,0.0009713048,0.00143438,0.004249099,0.04311043,0.007502599,0.04385909],"genre_scores_gemma":[0.5007247,0.006290109,0.3132408,0.0008906157,0.0002261356,0.00309814,0.09404911,0.002033478,0.07944695],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.008154293,"threshold_uncertainty_score":0.02727884,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1353396892443784,"score_gpt":0.4733861530791912,"score_spread":0.3380464638348127,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}