{"id":"W4384283659","doi":"10.1016/j.omtm.2023.07.002","title":"Inducible HEK293 AAV packaging cell lines expressing Rep proteins","year":2023,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université Laval; National Research Council Canada","funders":"Fonds de Recherche du Québec - Santé; National Research Council Canada; Natural Sciences and Engineering Research Council of Canada","keywords":"HEK 293 cells; Transfection; Cell culture; Biology; Transduction (biophysics); Plasmid; Titer; Molecular biology; Cell biology; Viral vector; Activator (genetics); Recombinant DNA; Gene; Virus; Virology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.007654297,0.0004808298,0.0005776571,0.0002258042,0.0003125804,0.0001230566,0.0007909892,0.0004704733,0.00009482424],"category_scores_gemma":[0.0006460651,0.0004425203,0.0003701996,0.0007258079,0.0001926054,0.00001056999,0.0005110311,0.0005566327,0.0001690549],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004732654,"about_ca_system_score_gemma":0.0006900493,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007553493,"about_ca_topic_score_gemma":0.000007921983,"domain_scores_codex":[0.9936521,0.002294803,0.00114969,0.001351067,0.0005989252,0.0009534325],"domain_scores_gemma":[0.9976107,0.0002120064,0.0002655532,0.001305151,0.0002451648,0.0003613843],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007886356,0.0001880076,0.0006162364,0.00002303283,0.0001684619,0.00004146589,0.0001418217,0.00006915537,0.7391518,0.000004115238,0.0004749453,0.2583323],"study_design_scores_gemma":[0.001483667,0.0009954135,0.001546173,0.0000449187,0.000009109289,0.000004026549,0.00006503984,0.00009748356,0.8934926,0.0003007846,0.1014366,0.0005241765],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9340464,0.002696395,0.06040714,0.0006204217,0.0004311342,0.0009453549,0.000004608313,0.0001630724,0.0006855032],"genre_scores_gemma":[0.2714606,0.004121861,0.7122535,0.004232904,0.000817132,0.0008348213,0.0003729409,0.0003900761,0.005516158],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.6625857,"threshold_uncertainty_score":0.9998026,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1353396892443784,"score_gpt":0.4733861530791912,"score_spread":0.3380464638348127,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}