{"id":"W4384626741","doi":"10.1002/cpz1.833","title":"Western Blotting with Fast SDS‐PAGE and Semi‐Dry Protein Transfer","year":2023,"lang":"en","type":"article","venue":"Current Protocols","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; McGill University Health Centre","funders":"","keywords":"Polyacrylamide gel electrophoresis; Chromatography; Lysis; Tris; Blot; Chemistry; Lysis buffer; Gel electrophoresis; Bradford protein assay; Sodium dodecyl sulfate; Gel electrophoresis of proteins; Protein purification; Molecular-weight size marker; Membrane protein; Polyacrylamide; Membrane; Biochemistry; Molecular biology; Biology; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001128401,0.0001929013,0.0001668565,0.00003920804,0.0001414572,0.00005932089,0.0001695829,0.0000728974,0.00007170174],"category_scores_gemma":[0.000007651867,0.0001652011,0.00003509124,0.0002009353,0.00006837789,0.0001401413,0.00007431093,0.000292443,0.00003059113],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000265914,"about_ca_system_score_gemma":0.00002704295,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000001639979,"about_ca_topic_score_gemma":0.000001528723,"domain_scores_codex":[0.9989457,0.000009981846,0.0002214711,0.0003319497,0.0001545453,0.000336394],"domain_scores_gemma":[0.9994546,0.0000192962,0.0000554123,0.0003253188,0.00003573066,0.0001096776],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001244154,0.0003416884,0.03925687,0.003210562,0.00003428329,0.000008591011,0.0007382825,0.00002006911,0.8145978,0.003443683,0.0001643468,0.1380594],"study_design_scores_gemma":[0.001199809,0.00007611639,0.0004683538,0.001796321,0.00001786399,0.000009813903,0.00009824504,0.0003362845,0.8366984,0.001304496,0.1573992,0.0005950783],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.7630478,0.0001054835,0.0754711,0.0003383726,0.00001721513,0.1546396,0.0001727641,0.002381846,0.003825855],"genre_scores_gemma":[0.2355384,0.00001320746,0.004818674,0.00001554718,0.0002038203,0.7580371,0.00006097909,0.00008121092,0.001230959],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.6033976,"threshold_uncertainty_score":0.6736706,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03581546124369796,"score_gpt":0.3408125752948959,"score_spread":0.304997114051198,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}