{"id":"W4384916116","doi":"10.2139/ssrn.4499951","title":"Strong Expression of Cas9 Under a New 3’-Truncated TEF1α Promoter Enhances CRISPR-Cas9-Mediated Genome Editing in <i>Yarrowia Lipolytica</i>","year":2023,"lang":"en","type":"preprint","venue":"SSRN Electronic Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"","keywords":"Yarrowia; CRISPR; Genome editing; Cas9; CRISPR interference; Biology; Genetics; Genome; Yeast; Computational biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004171865,0.0007186832,0.0008511636,0.0003761182,0.0003497469,0.0008996032,0.000807082,0.0009299461,0.003889779],"category_scores_gemma":[0.0003840135,0.000541287,0.00070649,0.0003216245,0.0006265996,0.0005035875,0.0009914809,0.002607916,0.004240818],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006893461,"about_ca_system_score_gemma":0.0004318982,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001155811,"about_ca_topic_score_gemma":0.00138822,"domain_scores_codex":[0.9994124,0.0000490994,0.00007869488,0.0002027686,0.0001559644,0.000100989],"domain_scores_gemma":[0.999632,0.00008083462,0.00008138753,0.00008780374,0.00003196521,0.0000860142],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004680159,0.00001252608,0.00005546796,0.00002944148,0.000004858213,0.0000436079,0.00001500466,0.00004305249,0.9987397,0.0001842815,0.00007839689,0.0007469983],"study_design_scores_gemma":[0.000008808291,0.00001699163,0.0006666107,0.000004653735,0.000009218808,0.0001345174,0.000009995295,0.0006864767,0.9949765,0.00005647349,0.003422297,0.000007462152],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.837831,0.001609534,0.1359539,0.0008047224,0.0006191854,0.0002654827,0.004743225,0.004580325,0.01359267],"genre_scores_gemma":[0.9404197,0.0004463569,0.02969195,0.0001845329,0.00003321805,0.0001168497,0.005256095,0.001241139,0.02261009],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003889779,"threshold_uncertainty_score":0.01301259,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01102005312549932,"score_gpt":0.2817813172903609,"score_spread":0.2707612641648616,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}