{"id":"W4385266044","doi":"10.1021/acschembio.3c00334","title":"Cellular Uptake of a Fluorescent Ligand Reveals Ghrelin <i>O</i>-Acyltransferase Interacts with Extracellular Peptides and Exhibits Unexpected Localization for a Secretory Pathway Enzyme","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Regulation of Appetite and Obesity","field":"Neuroscience","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Western University","funders":"Division of Chemistry; Congressionally Directed Medical Research Programs; Natural Sciences and Engineering Research Council of Canada; Syracuse University; U.S. Department of Education; National Science Foundation; National Institutes of Health; National Institute of General Medical Sciences; American Diabetes Association","keywords":"Ghrelin; Internalization; Extracellular; Cell biology; Biology; Peptide; Receptor; Peptide hormone; Intracellular; Ligand (biochemistry); Biochemistry; Acyltransferase; Signal transduction; Growth hormone secretagogue receptor; Enzyme; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000182618,0.0001793739,0.0002996481,0.00009436152,0.00006920634,0.00001647651,0.0001456522,0.0001739882,0.00001160369],"category_scores_gemma":[0.000410051,0.0001457291,0.00005615074,0.0003287821,0.0002933605,0.00008003871,0.00005224655,0.0001066667,0.000008401185],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000016016,"about_ca_system_score_gemma":0.00003648117,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005490474,"about_ca_topic_score_gemma":0.000002145898,"domain_scores_codex":[0.9986452,0.0000706275,0.0003869704,0.000485143,0.0001209528,0.0002910473],"domain_scores_gemma":[0.9990969,0.0003748212,0.000119056,0.0002092783,0.00009016487,0.0001098358],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002825283,0.00006515571,0.0003707326,0.00008960241,0.000007963062,0.000009724051,0.0002622684,0.00002377536,0.9967231,0.001202265,0.0001374296,0.0008255053],"study_design_scores_gemma":[0.0008677085,0.0002343714,0.0001630092,0.00008216228,0.00001372775,0.000007914938,0.00003739293,0.001194139,0.9941518,0.000838861,0.002245411,0.0001634728],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952669,0.0001773003,0.003690512,0.0001999974,0.00005505627,0.0004311818,0.00005811548,0.0000924109,0.00002849301],"genre_scores_gemma":[0.9989897,0.0001245543,0.0003416741,0.000187494,0.00006411885,0.0000336739,0.0001599538,0.00002206853,0.00007673856],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003722791,"threshold_uncertainty_score":0.594266,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02176322585804046,"score_gpt":0.2461612564572606,"score_spread":0.2243980305992201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}