{"id":"W4385266044","doi":"10.1021/acschembio.3c00334","title":"Cellular Uptake of a Fluorescent Ligand Reveals Ghrelin <i>O</i>-Acyltransferase Interacts with Extracellular Peptides and Exhibits Unexpected Localization for a Secretory Pathway Enzyme","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Regulation of Appetite and Obesity","field":"Neuroscience","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Western University","funders":"Division of Chemistry; Congressionally Directed Medical Research Programs; Natural Sciences and Engineering Research Council of Canada; Syracuse University; U.S. Department of Education; National Science Foundation; National Institutes of Health; National Institute of General Medical Sciences; American Diabetes Association","keywords":"Ghrelin; Internalization; Extracellular; Cell biology; Biology; Peptide; Receptor; Peptide hormone; Intracellular; Ligand (biochemistry); Biochemistry; Acyltransferase; Signal transduction; Growth hormone secretagogue receptor; Enzyme; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001052858,0.0001870206,0.0001802841,0.0000768376,0.0001493157,0.0001968908,0.0001976002,0.0002784087,0.0006055558],"category_scores_gemma":[0.00007572272,0.0001040802,0.0002029438,0.00008854931,0.0002024925,0.0001656804,0.0001720867,0.0003653597,0.000233547],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004799293,"about_ca_system_score_gemma":0.0001975437,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001922503,"about_ca_topic_score_gemma":0.002293445,"domain_scores_codex":[0.9999275,0.000009690736,0.000002414592,0.00001956604,0.00001404432,0.00002681384],"domain_scores_gemma":[0.9999664,0.00001067379,0.000006360692,0.000002754832,0.000004176762,0.000009734425],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003272224,0.000006301356,0.00008958258,0.000007514927,0.000002555482,0.00003643477,0.00001381898,0.00002814014,0.9994214,0.00011803,0.00001564347,0.0002279361],"study_design_scores_gemma":[0.000006531209,0.00008771173,0.002527698,0.000002098328,0.000009127125,0.0002143744,0.00003264907,0.001962252,0.9941944,0.00005496817,0.0009035118,0.000004668223],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935716,0.0003836535,0.004399561,0.0001213615,0.000008141821,0.00000565367,0.00006474122,0.00003138777,0.0014137],"genre_scores_gemma":[0.9931971,0.0003143726,0.004384826,0.0000591365,0.000003834192,0.00001322485,0.0002286444,0.00001864123,0.001780209],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001922503,"threshold_uncertainty_score":0.003822625,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02176322585804046,"score_gpt":0.2461612564572606,"score_spread":0.2243980305992201,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}