{"id":"W4385475937","doi":"10.1021/acschembio.3c00053","title":"A Fluorogenic Disaccharide Substrate for α-Mannosidases Enables High-Throughput Screening and Identification of an Inhibitor of the GH92 Virulence Factor from <i>Streptococcus pneumoniae</i>","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; University of Victoria; Simon Fraser University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Virulence; Virulence factor; Glycan; Disaccharide; Microbiology; Streptococcus pneumoniae; Biology; Bacteria; Glycosylation; Biochemistry; Glycoprotein; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001456784,0.0001233987,0.0001859696,0.00003312658,0.00006016411,0.00001266696,0.0002809096,0.0001991064,0.000007180151],"category_scores_gemma":[0.000344573,0.00009421288,0.00007612994,0.0001555555,0.0002924689,0.00001011889,0.0001978809,0.00007800577,0.000001876188],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000566986,"about_ca_system_score_gemma":0.0000409021,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001174525,"about_ca_topic_score_gemma":0.00002907045,"domain_scores_codex":[0.9988741,0.0000696985,0.000351504,0.0003873454,0.00009832483,0.0002190832],"domain_scores_gemma":[0.9991794,0.0001015102,0.0001770159,0.0003594841,0.0001253164,0.00005733044],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008312682,0.00003507267,0.006954284,0.00002738228,0.00003399654,2.076121e-7,0.00003273174,0.000005781714,0.9895561,0.00006910824,0.00004988987,0.003152322],"study_design_scores_gemma":[0.0004449033,0.0001267659,0.008382331,0.0000165109,0.00001193277,8.215361e-7,0.00005024289,0.0001169685,0.9896864,0.000701694,0.0003631137,0.00009829622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980439,0.0002176682,0.0002753295,0.0001687207,0.00005328199,0.0004063089,0.0008176738,0.00001361778,0.000003532617],"genre_scores_gemma":[0.9987573,0.0001121235,0.0001965369,0.00003305821,0.00009023325,0.00009410981,0.0006724869,0.00001321243,0.0000309576],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003054026,"threshold_uncertainty_score":0.384189,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01959888296840859,"score_gpt":0.2894090819063613,"score_spread":0.2698101989379528,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}