{"id":"W4385475937","doi":"10.1021/acschembio.3c00053","title":"A Fluorogenic Disaccharide Substrate for α-Mannosidases Enables High-Throughput Screening and Identification of an Inhibitor of the GH92 Virulence Factor from <i>Streptococcus pneumoniae</i>","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; University of Victoria; Simon Fraser University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Virulence; Virulence factor; Glycan; Disaccharide; Microbiology; Streptococcus pneumoniae; Biology; Bacteria; Glycosylation; Biochemistry; Glycoprotein; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003974161,0.0009633091,0.0003718554,0.0004240856,0.000230423,0.0002559474,0.0004949063,0.0005978648,0.001192126],"category_scores_gemma":[0.0002238665,0.0002658638,0.0003151994,0.0004762005,0.0002396304,0.0002233118,0.0002818599,0.0007629898,0.0005956423],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003603572,"about_ca_system_score_gemma":0.0002628619,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007367069,"about_ca_topic_score_gemma":0.001766145,"domain_scores_codex":[0.9996089,0.0001210475,0.00002167867,0.00006863367,0.0001099541,0.00006972932],"domain_scores_gemma":[0.9998385,0.00004875147,0.00003305737,0.00002629358,0.00002350916,0.00003000965],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008169039,0.00004767531,0.0001111953,0.0000457313,0.000006561275,0.00002939965,0.000008402714,0.0001429772,0.996351,0.00009423908,0.0001136146,0.002967505],"study_design_scores_gemma":[0.000009280553,0.0002489808,0.0005804231,0.000004510855,0.000008513212,0.0001331774,0.00000538459,0.0004409909,0.996681,0.00002306759,0.001859756,0.000004887254],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8382637,0.006556774,0.1415333,0.0005217017,0.0001084244,0.0004576753,0.002426922,0.00115666,0.008974873],"genre_scores_gemma":[0.8774406,0.005155598,0.1060521,0.0001884053,0.00003475145,0.0003976554,0.004443377,0.0001193148,0.006168257],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001192126,"threshold_uncertainty_score":0.003988028,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01959888296840859,"score_gpt":0.2894090819063613,"score_spread":0.2698101989379528,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}