{"id":"W4385663970","doi":"10.12688/f1000research.130126.2","title":"Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence","year":2023,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Signaling Pathways in Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Mitacs; National Institutes of Health; Ontario Genomics; Genome Canada","keywords":"Moesin; Western blot; Immunoprecipitation; Immunofluorescence; Antibody; Cytoskeleton; Cell biology; Blot; Actin cytoskeleton; Radixin; Biology; Ezrin; Molecular biology; Virology; Immunology; Cell; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001169195,0.0001808078,0.0002504159,0.0002033695,0.00008394597,0.0001024929,0.0004330607,0.0002621469,0.00000145224],"category_scores_gemma":[0.00149404,0.0001986371,0.00009599159,0.00009917993,0.0001831536,0.00001067877,0.0006284235,0.0001660933,0.000001886969],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003299265,"about_ca_system_score_gemma":0.0002253946,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003233957,"about_ca_topic_score_gemma":0.0001100291,"domain_scores_codex":[0.9982545,0.00009397403,0.0005208412,0.0005873009,0.000238378,0.0003050439],"domain_scores_gemma":[0.9982828,0.0002989083,0.0001909362,0.0006194153,0.0005595095,0.00004841735],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006468446,0.0000842576,0.03563153,0.001285859,0.0001000004,0.000001062201,0.0003403591,0.000715105,0.9588358,0.00006784249,0.0007415396,0.001549747],"study_design_scores_gemma":[0.0009450677,0.0001861219,0.08350646,0.0003712232,0.00003567292,9.004734e-7,0.0001444266,0.002424746,0.9093086,0.002612334,0.0001914527,0.0002729577],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9879737,0.001277789,0.007764981,0.00009998685,0.0002747704,0.001666925,0.0009226869,0.00001817413,9.644112e-7],"genre_scores_gemma":[0.9926478,0.0007032519,0.003097121,0.000008743953,0.000119803,0.0009603338,0.001447734,0.00006996242,0.0009452803],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04952722,"threshold_uncertainty_score":0.8100184,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06620591571009488,"score_gpt":0.3621808207981537,"score_spread":0.2959749050880588,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}