{"id":"W4385690508","doi":"10.1021/acschembio.3c00140","title":"Disrupting Protein Expression with Double-Clicked sgRNA–Cas9 Complexes: A Modular Approach to CRISPR Gene Editing","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Cas9; Guide RNA; CRISPR; Subgenomic mRNA; Genome editing; Biology; RNA; Computational biology; Gene; Endonuclease; Nucleotide; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001782933,0.0002667168,0.0002681845,0.00007058502,0.00009038118,0.00002210526,0.0003159539,0.0002659129,0.000005939421],"category_scores_gemma":[0.0000880984,0.0002212588,0.00007096164,0.0002753254,0.00009043368,0.000003811774,0.0003868548,0.0001786439,0.00002661303],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001572037,"about_ca_system_score_gemma":0.00002236005,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001867453,"about_ca_topic_score_gemma":0.000001654193,"domain_scores_codex":[0.998301,0.00003338261,0.0002763264,0.0006868642,0.0001179869,0.000584454],"domain_scores_gemma":[0.9992332,0.00001847694,0.00006428517,0.0004338241,0.00006693787,0.0001832033],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001408411,0.00004204145,0.0003554516,0.00004120234,0.00003251515,0.000003711262,0.00008511973,0.000466487,0.9976766,0.00006392734,0.0004293699,0.0006627236],"study_design_scores_gemma":[0.0008830067,0.0001564923,0.00009082282,0.00003183231,0.00001088961,0.00002770447,0.0001673612,0.0003425506,0.9930081,0.0000389973,0.00493537,0.0003069068],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.967594,0.0001316664,0.03091162,0.0001628819,0.00007056601,0.0004540664,0.0000212587,0.0001066644,0.0005473353],"genre_scores_gemma":[0.9856446,0.00001132186,0.0123847,0.0001388795,0.0006457942,0.0002492007,0.0007167111,0.00004958639,0.0001592351],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01852691,"threshold_uncertainty_score":0.9022672,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01774331278367182,"score_gpt":0.317570560014017,"score_spread":0.2998272472303452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}