{"id":"W4385690508","doi":"10.1021/acschembio.3c00140","title":"Disrupting Protein Expression with Double-Clicked sgRNA–Cas9 Complexes: A Modular Approach to CRISPR Gene Editing","year":2023,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Cas9; Guide RNA; CRISPR; Subgenomic mRNA; Genome editing; Biology; RNA; Computational biology; Gene; Endonuclease; Nucleotide; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002608103,0.0006208038,0.0004023557,0.0002869052,0.0001635874,0.0004155496,0.0006270378,0.0005861444,0.0007623244],"category_scores_gemma":[0.0002090626,0.0003071677,0.0004031605,0.0002391801,0.0004009123,0.0003259481,0.0005169513,0.001046865,0.0005924169],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004009224,"about_ca_system_score_gemma":0.0002438366,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002582377,"about_ca_topic_score_gemma":0.0003602112,"domain_scores_codex":[0.9996955,0.00002831473,0.00002402699,0.0001026848,0.0001104445,0.00003898068],"domain_scores_gemma":[0.9998506,0.00001948615,0.00005727346,0.00002232055,0.00001517504,0.00003501253],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000170782,0.00001318772,0.0000274808,0.00002309514,0.000004842552,0.00003269735,0.000005487776,0.0001302969,0.9981887,0.0002291228,0.00004592575,0.001282051],"study_design_scores_gemma":[0.000008822313,0.00007762692,0.00022447,0.000001799229,0.000006238572,0.0001127003,0.000002251687,0.001450509,0.9960986,0.00004876214,0.00196075,0.000007327939],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7303202,0.001660892,0.2601718,0.0004419373,0.0002477549,0.0003271608,0.0006575357,0.002391573,0.003781079],"genre_scores_gemma":[0.891498,0.00105374,0.1010685,0.0001677683,0.00003522912,0.0001781433,0.0005386078,0.0001441259,0.005316011],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007623244,"threshold_uncertainty_score":0.002908885,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01774331278367182,"score_gpt":0.317570560014017,"score_spread":0.2998272472303452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}