{"id":"W4385691312","doi":"10.1021/acs.est.3c02492","title":"Genetic Engineering of <i>Acidithiobacillus ferridurans</i> Using CRISPR Systems To Mitigate Toxic Release in Biomining","year":2023,"lang":"en","type":"article","venue":"Environmental Science & Technology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; University of Waterloo","funders":"Hospital for Sick Children; University of Toronto","keywords":"CRISPR; Acidithiobacillus; Cas9; Chemistry; Acidithiobacillus ferrooxidans; Computational biology; Gene; Materials science; Biology; Bioleaching; Metallurgy; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002163338,0.0001545743,0.0001644432,0.0006484939,0.00006393347,0.00001228491,0.000377459,0.0001325954,0.000004309422],"category_scores_gemma":[0.00006227793,0.0001715084,0.00003542397,0.001202342,0.0002592754,0.000006806704,0.0003165528,0.00009192611,0.0000164552],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008265823,"about_ca_system_score_gemma":0.00003309979,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000231447,"about_ca_topic_score_gemma":0.000005576659,"domain_scores_codex":[0.9986072,0.000009143912,0.0002676735,0.0004477703,0.0001947843,0.0004734377],"domain_scores_gemma":[0.9994847,0.000005650994,0.00004330207,0.000359154,0.000006540208,0.0001006556],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000004004354,0.00001235648,0.00580898,0.0000105697,0.000003580016,0.00001187743,0.00004432207,0.1644794,0.8287168,0.000008277075,0.000007723169,0.0008921325],"study_design_scores_gemma":[0.0002309694,0.0001533516,0.02001229,0.00004369938,0.000005997962,0.00005033878,0.0004107713,0.02244085,0.9555142,0.000005939645,0.0009029323,0.0002286772],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.991896,0.0005042242,0.00703204,0.00004963428,0.0002180188,0.0002183445,0.00001542054,0.00004506384,0.0000212784],"genre_scores_gemma":[0.9982535,0.00008174135,0.00154025,0.00001554378,0.00003058055,0.00002668411,0.000004563241,0.00002093687,0.00002617398],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1420386,"threshold_uncertainty_score":0.6993909,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006056774478126588,"score_gpt":0.2496458378345705,"score_spread":0.243589063356444,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}