{"id":"W4385704156","doi":"10.1007/978-1-0716-3397-7_10","title":"Measuring Protein–Protein Interactions in Cells using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay","year":2023,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Structural Genomics Consortium","funders":"","keywords":"Bioluminescence; Förster resonance energy transfer; Fusion protein; Protein–protein interaction; Chemistry; Green fluorescent protein; Biophysics; Transfection; Fluorescence; Cell biology; Biochemistry; Computational biology; Biology; Recombinant DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002343731,0.003049473,0.002648166,0.00170408,0.001737953,0.001948596,0.002434866,0.001608026,0.004551058],"category_scores_gemma":[0.001008579,0.001210195,0.002064173,0.002469121,0.001131477,0.001669167,0.001365747,0.005654377,0.00475367],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001303432,"about_ca_system_score_gemma":0.0006898012,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001190528,"about_ca_topic_score_gemma":0.002636319,"domain_scores_codex":[0.9953511,0.001092386,0.0004524111,0.001301232,0.001354958,0.0004479081],"domain_scores_gemma":[0.9989279,0.0004064297,0.0001314865,0.0002433276,0.0001990037,0.00009191029],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000110695,0.0001377517,0.0001359417,0.0001363929,0.00004497213,0.00002750303,0.00006031075,0.0001208563,0.9966397,0.0004123678,0.0003411171,0.001832208],"study_design_scores_gemma":[0.00001981651,0.00008187711,0.0006486761,0.00001105621,0.00002923712,0.00005797154,0.00001617778,0.002042589,0.9936608,0.0001257314,0.00328777,0.0000182037],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3246195,0.004590176,0.6388199,0.0008640548,0.0006757003,0.001039885,0.006357855,0.008054492,0.01497828],"genre_scores_gemma":[0.3287956,0.00557912,0.6055103,0.0009534197,0.0002173594,0.004577916,0.02185876,0.001541697,0.03096582],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004551058,"threshold_uncertainty_score":0.01522481,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05719757044039245,"score_gpt":0.3818454752464748,"score_spread":0.3246479048060823,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}