{"id":"W4385779963","doi":"10.1038/s42003-023-05191-5","title":"Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants","year":2023,"lang":"en","type":"article","venue":"Communications Biology","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Defense Science and Engineering Graduate; Division of Chemical, Bioengineering, Environmental, and Transport Systems; Agricultural Research Service; Office of Science; Gordon and Betty Moore Foundation; Foundation for Food and Agriculture Research; U.S. Department of Agriculture; Alfred P. Sloan Foundation; University of California Berkeley; Camille and Henry Dreyfus Foundation; National Science Foundation; National Institutes of Health; U.S. Department of Health and Human Services; Philomathia Foundation; U.S. Department of Energy; U.S. Department of Defense","keywords":"Nicotiana benthamiana; Bimolecular fluorescence complementation; mCherry; Complementation; Protein-fragment complementation assay; Recombinant DNA; Cell-penetrating peptide; Arabidopsis; Cytosol; Peptide; Cell biology; Luciferase; Biology; Biochemistry; Transfection; Green fluorescent protein; Yeast; Enzyme; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008695832,0.001084333,0.0006718103,0.001034526,0.0005577483,0.0008624911,0.000829726,0.0007627782,0.001929432],"category_scores_gemma":[0.0004638169,0.0003952335,0.0004734938,0.0009054414,0.001014837,0.0005633147,0.0008045611,0.002004167,0.000983292],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00143145,"about_ca_system_score_gemma":0.000604714,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002341615,"about_ca_topic_score_gemma":0.003039506,"domain_scores_codex":[0.9992035,0.0001600354,0.00005877857,0.0002281885,0.0002400176,0.0001096058],"domain_scores_gemma":[0.9992571,0.0002038433,0.0002093567,0.0001346305,0.00008349343,0.0001114874],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000186963,0.00001564348,0.00007232801,0.00003003188,0.000003549772,0.00001054175,0.00001395721,0.0000380641,0.9988562,0.0001278301,0.00003266836,0.0007805379],"study_design_scores_gemma":[0.0000055364,0.00005539826,0.0008903862,0.000003718637,0.000008590325,0.00009792358,0.0000120596,0.001187183,0.9964625,0.00004070194,0.001227859,0.000008104143],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7984291,0.00106006,0.1863954,0.0006122289,0.00007380127,0.0005574116,0.005393799,0.002635915,0.004842374],"genre_scores_gemma":[0.8642045,0.001249489,0.1211533,0.0002037901,0.00001963563,0.0007347012,0.005815671,0.0006200534,0.005998853],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002341615,"threshold_uncertainty_score":0.01038593,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1663150093782667,"score_gpt":0.3286222006649681,"score_spread":0.1623071912867014,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}