{"id":"W4385850442","doi":"10.1021/acssynbio.3c00170","title":"CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; Concordia University","keywords":"Metabolic engineering; Biology; Synthetic biology; Computational biology; Metabolic pathway; Genetics; DNA shuffling; Gene; Yeast; Saccharomyces cerevisiae; Directed evolution; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003932548,0.000257676,0.0002764649,0.0001152035,0.00008714919,0.00001367307,0.0003389193,0.0005108108,0.00006022541],"category_scores_gemma":[0.0003300179,0.0002335538,0.0001530683,0.0003331403,0.0001957586,0.000003305043,0.0002115485,0.0001545846,0.0001542635],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002051343,"about_ca_system_score_gemma":0.00008821549,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003899256,"about_ca_topic_score_gemma":0.00005974144,"domain_scores_codex":[0.9982011,0.0001406973,0.0003363299,0.0006640976,0.00008733536,0.000570477],"domain_scores_gemma":[0.999228,0.00005425615,0.00008658217,0.0004957447,0.0000380489,0.00009733876],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006335392,0.00008104656,0.003424158,0.00002753365,0.00005160658,0.00004004091,0.00002187605,0.000007229277,0.9893624,0.00009471463,0.0006472832,0.006178767],"study_design_scores_gemma":[0.0003987712,0.0001632901,0.006549955,0.00005806945,0.0000165976,0.0001190729,0.0001020747,0.0001125969,0.9431186,0.0001316617,0.04883739,0.0003919316],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9960185,0.0003444878,0.00003081347,0.0003714918,0.0002281468,0.000145675,0.0002129529,0.0001387921,0.002509186],"genre_scores_gemma":[0.9977055,0.0002685553,0.0001811592,0.0002010283,0.0002205957,0.00004949017,0.0005641828,0.0000276606,0.0007817807],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0481901,"threshold_uncertainty_score":0.952405,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01622160960687528,"score_gpt":0.2449964642775749,"score_spread":0.2287748546706996,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}