{"id":"W4385850442","doi":"10.1021/acssynbio.3c00170","title":"CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; Concordia University","keywords":"Metabolic engineering; Biology; Synthetic biology; Computational biology; Metabolic pathway; Genetics; DNA shuffling; Gene; Yeast; Saccharomyces cerevisiae; Directed evolution; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004567291,0.0008617945,0.0005172353,0.0006057702,0.0003829103,0.0007099285,0.0007225822,0.0003402134,0.001390759],"category_scores_gemma":[0.0006529761,0.0004019344,0.0005556785,0.0006128834,0.0004088026,0.0003047484,0.001031594,0.001033344,0.0007590465],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000507452,"about_ca_system_score_gemma":0.0008352351,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00368104,"about_ca_topic_score_gemma":0.003698633,"domain_scores_codex":[0.9997429,0.00002884514,0.00002442039,0.00007065149,0.00008625153,0.00004680555],"domain_scores_gemma":[0.9997167,0.00005720913,0.0000599083,0.00006991663,0.00002848151,0.00006775751],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008756751,0.0002639056,0.002050827,0.0003426,0.0001186228,0.0003297032,0.0001514835,0.01715449,0.9241211,0.002679913,0.002527717,0.04938395],"study_design_scores_gemma":[0.0001533732,0.0005472272,0.002202687,0.00003843287,0.00004476414,0.0005398184,0.00008464642,0.07781983,0.8976089,0.0008932914,0.01997805,0.00008908817],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7167118,0.001312902,0.2288945,0.0002774618,0.0001481315,0.0005674716,0.005150343,0.04312703,0.003810318],"genre_scores_gemma":[0.8014022,0.00118097,0.1839476,0.0001231555,0.0000152579,0.0005243717,0.006009277,0.002423421,0.004373769],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00368104,"threshold_uncertainty_score":0.007319212,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01622160960687528,"score_gpt":0.2449964642775749,"score_spread":0.2287748546706996,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}