{"id":"W4385860983","doi":"10.1016/j.sbi.2023.102673","title":"Absolute quantification of protein number and dynamics in single cells","year":2023,"lang":"en","type":"review","venue":"Current Opinion in Structural Biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Canadian Institutes of Health Research; Academy of Finland; Sigrid Juséliuksen Säätiö; National Science Foundation","keywords":"Protein dynamics; Biological system; Dynamics (music); Computational biology; Fluorescence correlation spectroscopy; Biophysics; Live cell imaging; Biology; Chemistry; Fluorescence; Cell; Protein structure; Physics; Genetics; Optics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001313494,0.000277701,0.0006658123,0.0001514389,0.00001679211,0.000007276479,0.0002351925,0.0004568014,0.000003366592],"category_scores_gemma":[0.00006372899,0.0002435183,0.00009194782,0.0002405691,0.0002202566,0.000004259758,0.0002054687,0.0002651193,0.000003994764],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009514923,"about_ca_system_score_gemma":0.00006650165,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002068655,"about_ca_topic_score_gemma":0.00002894921,"domain_scores_codex":[0.9983637,0.0001527117,0.0006568299,0.0005350566,0.00004446808,0.0002472818],"domain_scores_gemma":[0.9992447,0.00002431723,0.0003695798,0.0002936774,0.00003832457,0.00002943909],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00002604816,0.00003527926,0.0005261429,0.01264454,0.00002319788,3.553381e-7,0.00001554241,0.000001511149,0.04517521,0.002035945,0.0000710249,0.9394452],"study_design_scores_gemma":[0.0006607588,0.0004266556,0.0002798237,0.02158169,0.00004353934,0.00003387826,0.00004339698,0.0001615144,0.01785634,0.003093688,0.9544088,0.001409958],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.002492415,0.9939951,0.0005955715,0.000006113337,0.001233141,0.001338854,0.0003072353,0.00002131476,0.00001025226],"genre_scores_gemma":[0.0006263557,0.995246,0.001634506,8.288114e-7,0.00009784081,0.0001711261,0.002174709,0.00003305773,0.00001561148],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.9543377,"threshold_uncertainty_score":0.9930388,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07045465643926975,"score_gpt":0.4201654069365826,"score_spread":0.3497107504973128,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}