{"id":"W4386101783","doi":"10.1016/j.abb.2023.109727","title":"Expanding the β-substitution reactions of serine synthase through mutagenesis of aromatic active site residues","year":2023,"lang":"en","type":"article","venue":"Archives of Biochemistry and Biophysics","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia, Okanagan Campus; Okanagan University College; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Active site; Serine; Stereochemistry; Cysteine; Nucleophile; Pyridoxal; Enzyme; Biochemistry; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004709851,0.0005280999,0.0002935021,0.000256751,0.0002002652,0.0004522004,0.0004063441,0.000352871,0.001630128],"category_scores_gemma":[0.0005333105,0.0002610467,0.0003550082,0.0002833963,0.0003316828,0.0002254015,0.0003187332,0.0008408697,0.0005684805],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000337482,"about_ca_system_score_gemma":0.0005202296,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009203663,"about_ca_topic_score_gemma":0.00102148,"domain_scores_codex":[0.999747,0.00007808321,0.00005033164,0.00003798811,0.0000542392,0.00003243741],"domain_scores_gemma":[0.9996046,0.0001514413,0.0000938387,0.00005863932,0.00004348393,0.00004802718],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002282988,0.0001037367,0.0004811925,0.00005283577,0.00001515041,0.0001959709,0.00005044222,0.001014177,0.9914215,0.000706031,0.00003727351,0.005693275],"study_design_scores_gemma":[0.00002231674,0.0001791227,0.0006752583,0.00001031276,0.00002903006,0.0003595339,0.00002663257,0.001767084,0.9953353,0.0001280784,0.001456452,0.00001094167],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9888802,0.0002251555,0.009371608,0.00008828938,0.00003903597,0.00007579316,0.0001177747,0.00009107678,0.00111121],"genre_scores_gemma":[0.9936266,0.0002831399,0.005098179,0.00003419629,0.000005558735,0.00001828192,0.0001318535,0.00004462049,0.0007576606],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001630128,"threshold_uncertainty_score":0.005453348,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01315414873498641,"score_gpt":0.2489163860453643,"score_spread":0.2357622373103779,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}