{"id":"W4386214019","doi":"10.1016/j.foodcont.2023.110081","title":"Development of a novel visual assay for ultrasensitive detection of Listeria monocytogenes in milk and chicken meat harnessing helix loop-mediated isothermal amplification (HAMP)","year":2023,"lang":"en","type":"article","venue":"Food Control","topic":"Listeria monocytogenes in Food Safety","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"University of Veterinary and Animal Sciences; Indian Council of Agricultural Research","keywords":"Listeria monocytogenes; Loop-mediated isothermal amplification; Detection limit; Food science; Listeria; SYBR Green I; Chemistry; HAMP; Contamination; Biology; Microbiology; Chromatography; Bacteria; Molecular biology; Real-time polymerase chain reaction; Biochemistry; DNA; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007706929,0.00088758,0.0005389685,0.000521116,0.0002348175,0.0006736119,0.001055651,0.001174114,0.0006233458],"category_scores_gemma":[0.0007166721,0.0005666378,0.0004499129,0.00021336,0.0004867715,0.0005891669,0.0009261995,0.001088168,0.0005766189],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004416521,"about_ca_system_score_gemma":0.0004259564,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005194705,"about_ca_topic_score_gemma":0.000992285,"domain_scores_codex":[0.9990841,0.0001234865,0.00003884677,0.0003177527,0.0003324675,0.0001033468],"domain_scores_gemma":[0.9995221,0.0001507109,0.00009896357,0.00004754183,0.0001111163,0.00006947324],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002248665,0.00002182395,0.0001477136,0.00002994841,0.000004268252,0.00001802246,0.00001239373,0.00004806571,0.9964656,0.00006172142,0.00003129238,0.003136759],"study_design_scores_gemma":[0.00000670272,0.0001963957,0.0006004768,0.000004521097,0.00001242451,0.0001812501,0.00001210319,0.002960908,0.9949993,0.00003217447,0.0009808281,0.00001275966],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5579256,0.001921532,0.4343518,0.0003948181,0.000217132,0.0003910155,0.0003883681,0.002066343,0.002343289],"genre_scores_gemma":[0.720084,0.0008373812,0.2723396,0.0004367165,0.00004948795,0.0003058712,0.0006593656,0.0001114771,0.005176079],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001174114,"threshold_uncertainty_score":0.004075825,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05580453931318706,"score_gpt":0.3106448778595642,"score_spread":0.2548403385463771,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}