{"id":"W4386374683","doi":"10.1186/s40694-023-00165-y","title":"CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii","year":2023,"lang":"en","type":"article","venue":"Fungal Biology and Biotechnology","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Danmarks Tekniske Universitet","keywords":"Cellulase; Xylanase; Biology; Gene; Biorefinery; Microbiology; Thermophile; CRISPR; Overproduction; Repressor; Biochemistry; Transcription factor; Enzyme; Biotechnology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002064042,0.0001619196,0.0002421817,0.0003907329,0.00006388855,0.000003304546,0.00009559423,0.0007207467,0.00003071799],"category_scores_gemma":[0.00009415393,0.000145313,0.00004791555,0.000459995,0.0001864267,0.00005777204,0.00002447884,0.0002624982,0.00001808841],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002815426,"about_ca_system_score_gemma":0.000009616015,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001084941,"about_ca_topic_score_gemma":0.00001879314,"domain_scores_codex":[0.9989956,0.00003805426,0.000268924,0.0003468929,0.00004033851,0.0003102178],"domain_scores_gemma":[0.9996901,0.00003776745,0.00005943477,0.0001483356,0.00003391365,0.00003043357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007517733,0.00001941153,0.0005085579,0.0001712345,0.00002674817,0.000001321702,0.0001754085,0.00002084113,0.98522,0.00005003581,0.0001817311,0.01354958],"study_design_scores_gemma":[0.0005635973,0.0002087884,0.001931568,0.0000158379,0.00001170055,0.000008666211,0.0002769116,0.001042989,0.9929655,0.0001996608,0.002626923,0.0001479065],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9934217,0.0009018148,0.0007562618,0.00230727,0.00159814,0.0004191552,0.0000957002,0.0004874347,0.0000125766],"genre_scores_gemma":[0.9979779,0.001402825,0.0001681871,0.00002089099,0.0001966186,0.00003925989,0.0001354112,0.00001622552,0.00004274991],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01340167,"threshold_uncertainty_score":0.5925693,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01526873552725715,"score_gpt":0.2325900666896058,"score_spread":0.2173213311623487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}