{"id":"W4386374683","doi":"10.1186/s40694-023-00165-y","title":"CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii","year":2023,"lang":"en","type":"article","venue":"Fungal Biology and Biotechnology","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Danmarks Tekniske Universitet","keywords":"Cellulase; Xylanase; Biology; Gene; Biorefinery; Microbiology; Thermophile; CRISPR; Overproduction; Repressor; Biochemistry; Transcription factor; Enzyme; Biotechnology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001899471,0.0005010045,0.0003458656,0.0002281642,0.0001806799,0.0004200311,0.0004065572,0.0003953381,0.001568594],"category_scores_gemma":[0.0001813626,0.0001624587,0.0003307084,0.0001920826,0.0002197123,0.0001633865,0.0003408784,0.0007133455,0.0004746575],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002896067,"about_ca_system_score_gemma":0.0002429658,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001006369,"about_ca_topic_score_gemma":0.001323473,"domain_scores_codex":[0.9998243,0.00001788222,0.00002468883,0.00004951349,0.00005739777,0.00002620269],"domain_scores_gemma":[0.9998908,0.00002248337,0.00003850556,0.00001490032,0.00001268602,0.00002063664],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007818182,0.00002777166,0.000174822,0.00009054143,0.000009559301,0.0001923736,0.00003174968,0.0003307061,0.9962932,0.000262064,0.0001292777,0.002379764],"study_design_scores_gemma":[0.00003405862,0.0001323962,0.002445692,0.00002271339,0.00004989644,0.0004885985,0.0000487011,0.006978955,0.9795746,0.0001370522,0.01006837,0.00001895096],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9392413,0.001728454,0.04644456,0.0002936278,0.0001791996,0.0002275604,0.002891224,0.00138446,0.00760952],"genre_scores_gemma":[0.9682873,0.0008785661,0.01965349,0.00008682509,0.00001173412,0.0001200735,0.002098106,0.0001379085,0.00872582],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001568594,"threshold_uncertainty_score":0.005247414,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01526873552725715,"score_gpt":0.2325900666896058,"score_spread":0.2173213311623487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}