{"id":"W4386379060","doi":"10.7717/peerj.15539","title":"Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( <i>Paracentrotus lividus</i> ) oocytes","year":2023,"lang":"en","type":"article","venue":"PeerJ","topic":"Reproductive biology and impacts on aquatic species","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cryoprotectant; Paracentrotus lividus; Cryopreservation; Sea urchin; DNA damage; Biology; Vitrification; Oocyte; Seawater; Biochemistry; Andrology; Embryo; Cell biology; Ecology; DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003128737,0.000363142,0.0003685514,0.00019786,0.0001974753,0.0004154376,0.0004916178,0.0006273444,0.0006179076],"category_scores_gemma":[0.0006718382,0.0002437852,0.000407423,0.0001797721,0.00035441,0.0004712507,0.0002647657,0.0003493488,0.0001232504],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008801483,"about_ca_system_score_gemma":0.0006286069,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005906738,"about_ca_topic_score_gemma":0.004490492,"domain_scores_codex":[0.9999212,0.000009868442,0.000006050855,0.00002164914,0.00002697891,0.00001425426],"domain_scores_gemma":[0.9997846,0.00008895032,0.00004543363,0.00002056134,0.00004778585,0.00001265688],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"simulation_or_modeling","study_design_scores_codex":[0.00006613796,0.00003143671,0.001432752,0.0001925418,0.00001442875,0.0001137915,0.00008247441,0.8870943,0.1071993,0.0006838017,0.00008574935,0.00300337],"study_design_scores_gemma":[0.000007848814,0.0001010417,0.00103426,0.00001002077,0.00001289294,0.00002343104,0.00002749873,0.9584666,0.03961541,0.0002837525,0.0004067867,0.00001046297],"study_design_candidate":"simulation_or_modeling","study_design_consensus":"simulation_or_modeling","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8979386,0.0006169855,0.09759932,0.0001492542,0.00002032524,0.0001232681,0.0006337237,0.0001518857,0.002766647],"genre_scores_gemma":[0.9870951,0.0005217647,0.01114672,0.00001877984,0.000002491204,0.0001327831,0.0002077412,0.00003312293,0.0008415678],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005906738,"threshold_uncertainty_score":0.01174468,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01443114676153666,"score_gpt":0.2518672045965584,"score_spread":0.2374360578350218,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}