{"id":"W4386379060","doi":"10.7717/peerj.15539","title":"Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( <i>Paracentrotus lividus</i> ) oocytes","year":2023,"lang":"en","type":"article","venue":"PeerJ","topic":"Reproductive biology and impacts on aquatic species","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cryoprotectant; Paracentrotus lividus; Cryopreservation; Sea urchin; DNA damage; Biology; Vitrification; Oocyte; Seawater; Biochemistry; Andrology; Embryo; Cell biology; Ecology; DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002595136,0.000109651,0.0001759584,0.00007595013,0.00005441607,0.00001736192,0.00005333372,0.0001008253,0.00001615169],"category_scores_gemma":[0.0002549603,0.0001014956,0.00003040682,0.0001128899,0.0001189248,0.00002146311,0.00009437236,0.00006454928,6.849202e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008900071,"about_ca_system_score_gemma":0.00003266109,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001193817,"about_ca_topic_score_gemma":0.000002243871,"domain_scores_codex":[0.9991554,0.00008996841,0.0002351027,0.0002814669,0.0001082703,0.0001298013],"domain_scores_gemma":[0.9996183,0.00004974198,0.00009266446,0.0001635293,0.00003933065,0.00003645735],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001978686,0.00005393907,0.003042893,0.00003906518,0.00002441862,0.000001301183,0.0001625953,0.002797255,0.9935567,0.00008009904,0.00002291944,0.00002093253],"study_design_scores_gemma":[0.0003643057,0.0001523879,0.01774794,0.00002757742,0.000006165417,0.000007870289,0.0002715909,0.003454908,0.9777763,0.00008805165,0.00000642159,0.0000964292],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9989107,0.000431771,0.0001682316,0.0001249213,0.00003452109,0.0002363611,0.00004057003,0.000008515077,0.00004442812],"genre_scores_gemma":[0.9991872,0.0001256726,0.0004104647,0.00000818093,0.00006347243,0.00002923683,0.00007012569,0.000009814446,0.00009579396],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01578036,"threshold_uncertainty_score":0.4138872,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01443114676153666,"score_gpt":0.2518672045965584,"score_spread":0.2374360578350218,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}