{"id":"W4386430034","doi":"10.21769/bioprotoc.4805","title":"Isolation, Purification, and Culture of Embryonic Melanoblasts from Green Fluorescent Protein–expressing Reporter Mice","year":2023,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"melanin and skin pigmentation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Western University","funders":"Canadian Institutes of Health Research","keywords":"Cell sorting; Green fluorescent protein; Embryonic stem cell; Biology; Clonogenic assay; Molecular biology; Reporter gene; Cell biology; Melanocyte; Population; Cell culture; Gene expression; Gene; Flow cytometry; Genetics; Melanoma","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006540642,0.0008303124,0.0003610436,0.001081199,0.0009144681,0.0005530298,0.0006373273,0.0005664809,0.004907389],"category_scores_gemma":[0.0004313913,0.0007988045,0.0005213927,0.0005845936,0.0003820564,0.0003101075,0.0006366197,0.002117383,0.00584565],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004195215,"about_ca_system_score_gemma":0.0008502173,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001101557,"about_ca_topic_score_gemma":0.003965723,"domain_scores_codex":[0.9995346,0.00006874569,0.00006543102,0.00009906443,0.0001672276,0.00006506439],"domain_scores_gemma":[0.999521,0.00007667612,0.00007777812,0.0001249582,0.00009265804,0.0001068348],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005458761,0.00002417702,0.0001652377,0.00004961374,0.000004498614,0.00008940171,0.00003593125,0.000075099,0.9962969,0.0005017692,0.0005027942,0.002199955],"study_design_scores_gemma":[0.00004766076,0.0001475536,0.00364944,0.00004355873,0.00003174559,0.000623945,0.00003604354,0.0008245505,0.944599,0.0002742462,0.04970032,0.0000219193],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2833332,0.005201809,0.628548,0.001024058,0.0007547572,0.009002074,0.02633805,0.004451647,0.04134646],"genre_scores_gemma":[0.36998,0.008121099,0.468514,0.0007896539,0.0001431195,0.009859031,0.05462076,0.001905799,0.08606658],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004907389,"threshold_uncertainty_score":0.01641691,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01536599537972394,"score_gpt":0.2900609705416275,"score_spread":0.2746949751619036,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}