{"id":"W4386430034","doi":"10.21769/bioprotoc.4805","title":"Isolation, Purification, and Culture of Embryonic Melanoblasts from Green Fluorescent Protein–expressing Reporter Mice","year":2023,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"melanin and skin pigmentation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Western University","funders":"Canadian Institutes of Health Research","keywords":"Cell sorting; Green fluorescent protein; Embryonic stem cell; Biology; Clonogenic assay; Molecular biology; Reporter gene; Cell biology; Melanocyte; Population; Cell culture; Gene expression; Gene; Flow cytometry; Genetics; Melanoma","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001450747,0.0001227054,0.0001103731,0.00003956421,0.00007474978,0.00002826122,0.000101916,0.0001468128,0.00002994257],"category_scores_gemma":[0.00004299582,0.0001057815,0.00004042934,0.0001104124,0.00004937633,0.000008349411,0.00006461808,0.00004489554,0.00001070079],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008928598,"about_ca_system_score_gemma":0.00004247048,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005192856,"about_ca_topic_score_gemma":0.00001575683,"domain_scores_codex":[0.9990286,0.00006274166,0.0002955028,0.0003280663,0.0001514777,0.0001335857],"domain_scores_gemma":[0.9993169,0.000004863861,0.0002306456,0.0002853481,0.0001096406,0.00005265078],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001130837,0.0000273999,0.004127212,0.00008484335,0.00002370775,0.000001805237,0.0002344154,0.000001985657,0.987438,0.00001430125,0.006938731,0.0009945764],"study_design_scores_gemma":[0.0009495667,0.0001419344,0.01686947,0.00007018047,0.000009864509,0.000003792213,0.00009784481,0.0001600591,0.9424395,0.0002362195,0.03887352,0.0001480629],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.652099,0.00002933521,0.001964885,0.000389422,0.00009134573,0.3445945,0.00007067003,0.0000705436,0.0006902745],"genre_scores_gemma":[0.3069426,0.000004939373,0.005237788,0.0001013315,0.0005002539,0.6829314,0.0007622,0.00004495666,0.003474548],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.3451564,"threshold_uncertainty_score":0.4313644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01536599537972394,"score_gpt":0.2900609705416275,"score_spread":0.2746949751619036,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}