{"id":"W4386442673","doi":"10.1101/2023.09.01.555973","title":"Understanding the biosynthesis of human IgMs through a combinatorial expression of mutant subunits that affect different assembly steps","year":2023,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"","keywords":"Secretion; Protein subunit; Mutant; Biosynthesis; Biochemistry; HEK 293 cells; Mutagenesis; Secretory pathway; Cell biology; Biology; Chemistry; Enzyme; Cell; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000186682,0.000270786,0.0001651706,0.00013037,0.00009877478,0.0003650347,0.0001919952,0.0002537632,0.0003541001],"category_scores_gemma":[0.0001201974,0.0001209925,0.0002497482,0.0001198417,0.000174203,0.0001935554,0.0001726311,0.0004856923,0.000145584],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002591182,"about_ca_system_score_gemma":0.0002075591,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003785417,"about_ca_topic_score_gemma":0.0004268806,"domain_scores_codex":[0.9998761,0.00001934333,0.00002067012,0.00002965054,0.00003539964,0.00001877216],"domain_scores_gemma":[0.9999231,0.00001149392,0.00002916368,0.00001379963,0.000008747542,0.00001366878],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003193951,0.00001007563,0.0001824035,0.000009658635,0.000002970438,0.0000220454,0.000005243737,0.0002040009,0.9989704,0.00007721593,0.000007614313,0.0004764468],"study_design_scores_gemma":[0.000005164964,0.0001174176,0.001427834,0.00000289425,0.00001702197,0.0001687461,0.00002176023,0.004469538,0.992841,0.00007327273,0.000851035,0.000004270707],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9908344,0.0002966978,0.008171019,0.00007261088,0.00001051354,0.00001872311,0.0001768104,0.00005133218,0.0003679463],"genre_scores_gemma":[0.990827,0.0003148255,0.008114775,0.0000412439,0.0000044627,0.00001638725,0.0002517997,0.00001931216,0.0004101493],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0003785417,"threshold_uncertainty_score":0.00188005,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1392111931957139,"score_gpt":0.3154830302528285,"score_spread":0.1762718370571146,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}