{"id":"W4386476484","doi":"10.1016/j.ab.2023.115312","title":"Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization (ESI) for protein heterogeneity characterization","year":2023,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"Advanced Electrophoresis Solutions (Canada)","funders":"FedDev Ontario","keywords":"Chemistry; Electrospray ionization; Chromatography; Repeatability; Tandem mass spectrometry; Tandem; Isoelectric focusing; Electrospray; Mass spectrometry; Capillary electrophoresis; Analytical Chemistry (journal); Materials science; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004731253,0.000254091,0.0002365582,0.0001127299,0.0002902249,0.00007809917,0.0002015483,0.0003636908,0.00002136008],"category_scores_gemma":[0.0005662991,0.000272896,0.0001670865,0.0008545446,0.00009770488,0.00001684914,0.00006055651,0.0001389283,0.00001080069],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000163636,"about_ca_system_score_gemma":0.0001996755,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000757328,"about_ca_topic_score_gemma":0.000001568904,"domain_scores_codex":[0.9980176,0.00008547981,0.0004136586,0.0007169026,0.0002498883,0.0005164751],"domain_scores_gemma":[0.9989128,0.00001658919,0.0001820979,0.0004817196,0.0002677167,0.0001390795],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000904153,0.00006224189,0.0009274616,0.0000702189,0.00005462291,0.000001230004,0.000002948812,0.00002716886,0.9982361,0.00011079,0.0000564779,0.0003603908],"study_design_scores_gemma":[0.0004709676,0.000115129,0.001980757,0.0000140615,0.0000476247,0.000006584142,0.000007564513,0.01242126,0.9839715,0.0002433075,0.0004104919,0.0003107034],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8175821,0.0001788927,0.1812417,0.0002521923,0.00005054324,0.0005162019,0.00005879563,0.00008043889,0.0000391292],"genre_scores_gemma":[0.9930245,0.00006826768,0.003198974,0.00008300256,0.0003878016,0.00007759879,0.002735244,0.00004069928,0.0003838758],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1780428,"threshold_uncertainty_score":0.9999723,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01531833393180255,"score_gpt":0.2669692562213237,"score_spread":0.2516509222895212,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}