{"id":"W4386520053","doi":"10.1016/j.mcpro.2023.100644","title":"Pulse-SILAC and Interactomics Reveal Distinct DDB1-CUL4–Associated Factors, Cellular Functions, and Protein Substrates","year":2023,"lang":"en","type":"article","venue":"Molecular & Cellular Proteomics","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"DDB1; Stable isotope labeling by amino acids in cell culture; Ubiquitin; Cullin; Ubiquitin-Protein Ligases; Ubiquitin ligase; F-box protein; Biochemistry; Cell division control protein 4; Proteasome; Protein degradation; Chemistry; Ubiquitin-conjugating enzyme; Cell biology; Biology; Proteomics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004228147,0.0007149704,0.0005697289,0.00136755,0.0005803489,0.0008420247,0.0004528657,0.0007151114,0.001883204],"category_scores_gemma":[0.0002534099,0.0003722526,0.0006400833,0.0009331039,0.0003325976,0.0005474347,0.00052646,0.001156605,0.001252365],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007392992,"about_ca_system_score_gemma":0.0003171948,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001582371,"about_ca_topic_score_gemma":0.002117791,"domain_scores_codex":[0.9995467,0.00002657375,0.00002302557,0.0001485884,0.0001425404,0.000112563],"domain_scores_gemma":[0.9998242,0.00002703579,0.00003596999,0.00002065199,0.00004642396,0.00004562683],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002153051,0.00003279974,0.001716307,0.0001096839,0.00005941288,0.00009189935,0.00005545743,0.0001154288,0.9937772,0.0002172221,0.0003577725,0.003251505],"study_design_scores_gemma":[0.0000177124,0.00009399135,0.05748034,0.00001867237,0.00009690654,0.0007302918,0.0001497726,0.009215371,0.9209222,0.0004182917,0.01081331,0.00004302023],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9261484,0.008344346,0.0482044,0.0007594168,0.0001723154,0.00009555565,0.009023133,0.001370484,0.005881836],"genre_scores_gemma":[0.9040445,0.005268152,0.05725097,0.001288471,0.00008067852,0.0003156866,0.01674262,0.0005616431,0.01444733],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001883204,"threshold_uncertainty_score":0.006299973,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01183512832779534,"score_gpt":0.2181980030361695,"score_spread":0.2063628747083741,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}