{"id":"W4387245992","doi":"10.1021/acs.langmuir.3c02208","title":"Electrostatic-Mediated Binding of DNA to Lysozymes: Evaluation of Aptamer-Based Assays for Highly Positively Charged Targets","year":2023,"lang":"en","type":"article","venue":"Langmuir","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; China Scholarship Council","keywords":"Aptamer; DNA; Lysozyme; Chemistry; Biosensor; Fluorescence anisotropy; Fluorescence; Biophysics; Biochemistry; Molecular biology; Biology; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001237684,0.001124623,0.0006444121,0.0007083649,0.0003077357,0.0004682602,0.0007436508,0.00135674,0.0004847222],"category_scores_gemma":[0.001410492,0.000358226,0.0003919678,0.0006278107,0.0004554965,0.0004728389,0.0005641489,0.0006518918,0.0002975034],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003547337,"about_ca_system_score_gemma":0.0002690712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005346269,"about_ca_topic_score_gemma":0.0008869464,"domain_scores_codex":[0.9977995,0.000801903,0.0001271009,0.0003497883,0.000756501,0.0001652237],"domain_scores_gemma":[0.9990392,0.0003365275,0.0001944713,0.00004816188,0.0002557406,0.0001260449],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003265712,0.00003545708,0.0001107035,0.00005629272,0.000007913883,0.00001697638,0.00002533888,0.0001122548,0.9984592,0.00001741344,0.000008230518,0.001117653],"study_design_scores_gemma":[0.000007713843,0.0003202199,0.0007223196,0.000004419358,0.00001430556,0.00009089272,0.00002530865,0.001642057,0.9969144,0.0000221537,0.0002245336,0.00001171781],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9508243,0.004002338,0.04348416,0.0001974363,0.00007948941,0.0002643479,0.0002014815,0.0001513675,0.0007951633],"genre_scores_gemma":[0.9374866,0.00296466,0.05636722,0.0001511657,0.00005253594,0.0003291304,0.0002713535,0.00003245259,0.002344749],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00135674,"threshold_uncertainty_score":0.006545603,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02435860127938959,"score_gpt":0.3238605355170156,"score_spread":0.299501934237626,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}