{"id":"W4387365784","doi":"10.1128/spectrum.05356-22","title":"Design of a novel affinity probe using the cell wall-binding domain of a <i>Listeria monocytogenes</i> autolysin for pathogen detection","year":2023,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Listeria monocytogenes in Food Safety","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; Canadian Food Inspection Agency","funders":"Canadian Food Inspection Agency","keywords":"Listeria monocytogenes; Autolysin; Listeria; Microbiology; Fluorescence microscope; Biology; Chemistry; Immunogold labelling; Biochemistry; Molecular biology; Bacteria; Mutant; Fluorescence; Genetics; Antibody; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002004136,0.0005735759,0.0004097905,0.0001841649,0.0002018359,0.0002960578,0.0005735849,0.0006671139,0.000750111],"category_scores_gemma":[0.0002000102,0.000262906,0.0003214589,0.0001597478,0.000272124,0.0002397884,0.0002907069,0.000495129,0.0006060168],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005879965,"about_ca_system_score_gemma":0.0002503664,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006152045,"about_ca_topic_score_gemma":0.0004995213,"domain_scores_codex":[0.9998011,0.00003745676,0.00001251871,0.00006548849,0.00004476668,0.00003864034],"domain_scores_gemma":[0.9998208,0.00003137171,0.00004675962,0.00001475785,0.00003930291,0.00004711899],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001735289,0.00001047871,0.00005803404,0.00001747869,0.000001955688,0.00001221797,0.000004024674,0.00004215343,0.9994171,0.00003268439,0.00001364652,0.0003729298],"study_design_scores_gemma":[0.00001024754,0.000128504,0.0007018468,0.000002951279,0.000007174909,0.0001204407,0.000009164918,0.001812349,0.9960774,0.00001579478,0.001108081,0.000006174832],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9530537,0.0004952434,0.04454024,0.0002228976,0.0000361479,0.0001247374,0.000201828,0.0002653662,0.001059735],"genre_scores_gemma":[0.9488265,0.0002356547,0.04758269,0.0002720732,0.00001415551,0.0001580353,0.000611327,0.00003320291,0.00226638],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000750111,"threshold_uncertainty_score":0.004266262,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08684737789201116,"score_gpt":0.3041123670490529,"score_spread":0.2172649891570418,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}