{"id":"W4387370064","doi":"10.21769/bioprotoc.4838","title":"Fluorescence Resonance Energy Transfer to Detect Plasma Membrane Perturbations in Giant Plasma Membrane Vesicles","year":2023,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"PROTEO; Université du Québec à Montréal","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada","keywords":"Förster resonance energy transfer; Membrane; Vesicle; Biophysics; Chemistry; Liposome; Fluorescence; Peptide; Phospholipid; Membrane biophysics; Biological membrane; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004265757,0.0003489777,0.0003058751,0.0003292054,0.0001688553,0.0002702245,0.0003891599,0.0008842549,0.0008544356],"category_scores_gemma":[0.0004585635,0.0001823675,0.0002211073,0.0002514456,0.00021094,0.0004290733,0.0003826987,0.000694369,0.0004184961],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003263191,"about_ca_system_score_gemma":0.0001521711,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000276819,"about_ca_topic_score_gemma":0.000290124,"domain_scores_codex":[0.9997321,0.0000770949,0.00001292579,0.00006981251,0.00007082694,0.00003734781],"domain_scores_gemma":[0.9998043,0.00008380327,0.00003882853,0.0000212793,0.00003359106,0.00001817011],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001840781,0.00001313703,0.00004944585,0.00003018514,0.000002723693,0.00002435729,0.00001218626,0.0001434783,0.9984671,0.0001128398,0.00003402569,0.001091914],"study_design_scores_gemma":[0.000008089923,0.0001065336,0.0004551825,0.000005840204,0.000004176876,0.0001193901,0.00001959387,0.004086047,0.9943553,0.0001432507,0.0006901939,0.000006422081],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8620248,0.002292221,0.1316323,0.0002445196,0.0000758879,0.0001864112,0.0004615888,0.0006432626,0.002439039],"genre_scores_gemma":[0.9410852,0.001346988,0.05451326,0.0001327337,0.00001386074,0.0002183248,0.0003865678,0.00007424728,0.002228755],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008842549,"threshold_uncertainty_score":0.002858341,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01604817204181946,"score_gpt":0.2725804150060592,"score_spread":0.2565322429642398,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}