{"id":"W4387375963","doi":"10.3389/fimmu.2023.1275413","title":"Efficient and stable CRISPR/Cas9-mediated genome-editing of human type 2 innate lymphoid cells","year":2023,"lang":"en","type":"article","venue":"Frontiers in Immunology","topic":"IL-33, ST2, and ILC Pathways","field":"Immunology and Microbiology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Toronto General Hospital; University of Toronto; University Health Network","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Blood Services","keywords":"CRISPR; Genome editing; Cas9; Biology; Nucleofection; Innate lymphoid cell; Gene knockout; Computational biology; Innate immune system; Immune system; Genetics; Transfection; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006472897,0.0004106256,0.0004467212,0.0003069133,0.0003308488,0.0006926529,0.0005978298,0.0006252764,0.001448708],"category_scores_gemma":[0.0005221449,0.0003058054,0.0005215761,0.000243021,0.0004039201,0.0002323665,0.0004871571,0.0009980729,0.0008007509],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004680594,"about_ca_system_score_gemma":0.0006716771,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001141483,"about_ca_topic_score_gemma":0.002342633,"domain_scores_codex":[0.9991811,0.0001003697,0.00008488353,0.0002262035,0.0003120838,0.00009528042],"domain_scores_gemma":[0.999731,0.00006683878,0.00006204564,0.00006037753,0.00004083252,0.00003880396],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008815516,0.00003327142,0.0002782965,0.0001248482,0.00001716866,0.0001916878,0.00007140302,0.0009623037,0.9898461,0.0007976614,0.0006580331,0.006931128],"study_design_scores_gemma":[0.00002174,0.0001345411,0.001135734,0.00002644127,0.00002618226,0.0005630229,0.00002857009,0.004022828,0.9699822,0.0001963694,0.02383577,0.00002663579],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5780931,0.00405528,0.3913934,0.0007905834,0.0004735423,0.001444125,0.007076573,0.003531637,0.01314181],"genre_scores_gemma":[0.802671,0.002608362,0.1748285,0.0004382006,0.0000456913,0.001011265,0.005034705,0.0005313288,0.01283093],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001448708,"threshold_uncertainty_score":0.004846454,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0100583333234908,"score_gpt":0.2183590339245495,"score_spread":0.2083007006010587,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}