{"id":"W4387473048","doi":"10.2144/btn-2023-0042","title":"Playback Cloning: Simple, Reversible, Cost-effective Cloning for the Combinatorial Assembly of Complex Expression Constructs","year":2023,"lang":"en","type":"article","venue":"BioTechniques","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Brock University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cloning (programming); Computer science; Computational biology; Plasmid; Construct (python library); Usability; Multiple cloning site; Cloning vector; Biology; Expression vector; Recombinant DNA; Vector (molecular biology); Genetics; DNA; Programming language; Human–computer interaction; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003052171,0.0001457291,0.0001715944,0.00005596859,0.0001210791,0.00001743254,0.0002141338,0.0002090607,0.000004442242],"category_scores_gemma":[0.0001783516,0.0001183591,0.0001084626,0.0001606359,0.00008800792,0.00000337595,0.0001587182,0.00009211501,0.000001214658],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001280713,"about_ca_system_score_gemma":0.0000331889,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001891448,"about_ca_topic_score_gemma":0.000003593487,"domain_scores_codex":[0.999182,0.0000389661,0.0001939628,0.0002459712,0.0001087392,0.0002303249],"domain_scores_gemma":[0.9993237,0.0001436948,0.00009065676,0.0002989655,0.0001008474,0.00004214536],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008649155,0.000011881,0.0001905478,0.00004631724,0.00003293366,6.758124e-7,0.00003442963,0.000119585,0.9794469,0.000372701,0.01623391,0.003423661],"study_design_scores_gemma":[0.0004413656,0.0002736547,0.001220182,0.0000385222,0.00001895507,0.000003637711,0.00007281053,0.0005861294,0.9269329,0.0001821648,0.07009168,0.0001380497],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7361723,0.0008596679,0.2552864,0.0002609192,0.0009686922,0.00506656,0.0002406266,0.0003411768,0.0008036334],"genre_scores_gemma":[0.9944281,0.0002029234,0.004625347,0.00003856905,0.0002119487,0.0002613455,0.0001582727,0.00002673739,0.00004675111],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2582558,"threshold_uncertainty_score":0.4826542,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02257171078814457,"score_gpt":0.3474505850405466,"score_spread":0.324878874252402,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}