{"id":"W4387515915","doi":"10.1101/pdb.prot108340","title":"Validating Single-Guide RNA for<i>Aedes aegypti</i>Gene Editing","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Aedes aegypti; Computational biology; RNA editing; Gene; Biology; RNA; Computer science; Genetics; Ecology; Larva","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003410856,0.0009319178,0.0004652863,0.0006778661,0.0007686818,0.0008191445,0.0008976379,0.0008780851,0.003993936],"category_scores_gemma":[0.00229751,0.0008085191,0.0007964665,0.0004325245,0.0006999256,0.0005503871,0.0005989573,0.001585987,0.003424443],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009083062,"about_ca_system_score_gemma":0.001368374,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002745316,"about_ca_topic_score_gemma":0.005808783,"domain_scores_codex":[0.9977887,0.0004824919,0.0003690629,0.0004049587,0.0007766158,0.0001781266],"domain_scores_gemma":[0.9976346,0.0005843907,0.0004364764,0.0005739151,0.0006504777,0.0001200386],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003563334,0.00002430196,0.0002085216,0.00006944759,0.00000827376,0.00005198429,0.00003994145,0.0002611513,0.9943869,0.0006067155,0.0006253046,0.003681751],"study_design_scores_gemma":[0.00001027116,0.0000759705,0.0006227525,0.00001920753,0.00001256315,0.0001508579,0.00002015313,0.0008055013,0.9895561,0.00009491786,0.008620054,0.00001174476],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3494478,0.001233882,0.5957484,0.001218711,0.0007021116,0.00299975,0.02004909,0.004916538,0.02368377],"genre_scores_gemma":[0.392644,0.002775796,0.5354742,0.001009775,0.00005902468,0.002772588,0.03488523,0.003366104,0.0270133],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003993936,"threshold_uncertainty_score":0.01803857,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02819755587767136,"score_gpt":0.3395659930643332,"score_spread":0.3113684371866618,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}