{"id":"W4387711907","doi":"10.17504/protocols.io.8epv5xn66g1b/v1","title":"Optimized QIAGEN DNeasy Blood & Tissue kit Protocol for Environmental DNA Extraction v1","year":2023,"lang":"en","type":"preprint","venue":"","topic":"Environmental DNA in Biodiversity Studies","field":"Environmental Science","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Fisheries and Oceans Canada","funders":"","keywords":"Extraction (chemistry); Protocol (science); DNA extraction; Chemistry; DNA; Chromatography; Biochemistry; Medicine; Polymerase chain reaction; Pathology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003293255,0.003528978,0.003341577,0.003589838,0.002223923,0.002078032,0.002981033,0.001686552,0.04405213],"category_scores_gemma":[0.00378118,0.001655915,0.001734977,0.003255973,0.001290462,0.001002781,0.002004646,0.004619581,0.06002193],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007140505,"about_ca_system_score_gemma":0.003191463,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001992033,"about_ca_topic_score_gemma":0.005683834,"domain_scores_codex":[0.9964485,0.0006316481,0.0004131834,0.0009676439,0.001112064,0.0004269905],"domain_scores_gemma":[0.9986457,0.0003194867,0.00009826923,0.0004271178,0.000404243,0.0001052282],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0005935428,0.0004106871,0.001182844,0.005098018,0.0001736632,0.001099204,0.0007985863,0.001117327,0.8204216,0.005951752,0.08893603,0.07421663],"study_design_scores_gemma":[0.0001325628,0.000482608,0.003452921,0.0004736923,0.0002852761,0.001642155,0.0001318172,0.001538289,0.2295839,0.003158949,0.7588998,0.0002181559],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.02803645,0.007447491,0.797778,0.000901851,0.001968744,0.01068107,0.08683901,0.02579878,0.04054864],"genre_scores_gemma":[0.01711678,0.005318495,0.686217,0.001203031,0.0003771696,0.01337471,0.2048849,0.008687286,0.06282056],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.04405213,"threshold_uncertainty_score":0.147369,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06451553302785026,"score_gpt":0.3144805857581228,"score_spread":0.2499650527302726,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}